| 超临界二氧化碳提取物中生育酚的LC/APCI-MS2测定 |
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| 引用本文： | 周泉城,盛桂华,吴谋成. 超临界二氧化碳提取物中生育酚的LC/APCI-MS2测定[J]. 农业工程学报, 2004, 20(6): 212-215 |
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| 作者姓名： | 周泉城 盛桂华 吴谋成 |
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| 作者单位： | 山东理工大学轻工与农业工程学院食品系,淄博,250049;山东淄博矿业集团有限公司,淄博,255120;华中农业大学食品科学技术学院,武汉,430070 |
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| 基金项目： | the "Tenth Five-Year P1an" Science and Technology Key Project(2001BA501A20) |
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| 摘 要： | 确定了一种简单,明确和灵敏的高效液相测定和检测超临界二氧化碳萃取物中生育酚的方法。通过加入携带剂,超临界二氧化碳从菜籽脱臭馏出物提取生育酚浓缩物,其分析在反向色谱柱Zorbax C18上,用98％甲醇作为流动相,UV检测波长为292nm,α-生育酚作标准物。此方法线性相关性较高。APCI-MS和APCI-MS2检测的各生育酚的m/e与理论预测值一致。
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| 关 键 词： | 超临界二氧化碳 生育酚 LC/APCI-MS2 提取方法 食品分析 结构鉴定 |
| 收稿时间： | 2004-07-16 |
Detection of tocopherols in extracts by supercritical carbon dioxide with LC/APCI-MS2 |
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| Zhou Quancheng,Sheng Guihua and Wu Moucheng. Detection of tocopherols in extracts by supercritical carbon dioxide with LC/APCI-MS2[J]. Transactions of the Chinese Society of Agricultural Engineering, 2004, 20(6): 212-215 |
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| Authors: | Zhou Quancheng Sheng Guihua Wu Moucheng |
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| Abstract: | A simple, specific and sensitive high-performance liquid chromatographic method was developed for the determination and detection of tocopherols in extracts by supercritical carbon dioxide. A supercritical carbon dioxide extraction with cosolvents procedure was used to concentrate tocopherols from the rapeseed deodorize distillates. The analyses were separated on a Zorbax C18 reversed-phase column using 98% methanol as mobile phase, UV detection at 292 nm and α-tocopherol as a standard. The calibration graphs of the method were linear. Results by APCI-MS2 experiments were consistent with outcomes from theories. |
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| Keywords: | supercritical carbon dioxide tocopherols LC/APCI-MS2 extraction methods food analysis structure identification |
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