| 鸡传染性支气管炎病毒(IBV)结构蛋白的研究 |
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| 引用本文： | 陆苹,孙建和,李晖,王欣,孙涛,张红燕,王国丰. 鸡传染性支气管炎病毒(IBV)结构蛋白的研究[J]. 上海交通大学学报(农业科学版), 2000, 18(1): 1-3 |
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| 作者姓名： | 陆苹 孙建和 李晖 王欣 孙涛 张红燕 王国丰 |
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| 作者单位： | 上海交通大学,上海市兽医生物枝术重点实验室,上海,201101 |
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| 基金项目： | 上海市科技发展基金(963213012) |
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| 摘 要： | 应用SDS-PAGE对病毒的结构蛋白研究结果显示T株病毒与M41和H
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| 关 键 词： | 鸡肾型传染性支气管炎病毒；结构蛋白；SDS-PACE；免疫转印 |
| 文章编号： | 1000-193X(2000)-010001-03 |
| 修稿时间： | 2000-01-20 |
Analysis of structural polypeptide of IBV |
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| LU Ping,SUN Jian-he,LI Hui,WANG Xin,SUN Tao,ZHANG Hong-yan,WANG Guo-feng. Analysis of structural polypeptide of IBV[J]. Journal of Shanghai Jiaotong University (Agricultural Science), 2000, 18(1): 1-3 |
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| Authors: | LU Ping SUN Jian-he LI Hui WANG Xin SUN Tao ZHANG Hong-yan WANG Guo-feng |
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| Affiliation: | LU Ping ,SUN Jian-he ,LI Hui ,WANGXin ,SUN Tao ,ZHANG Hong-yan ;(Key Lab of Veterinary Bio-technology, Shanghai Jiaotong Univ.,Shanghai 201101,China) |
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| Abstract: | IBV were amplified and purified by ultracentrifugation,The proteins were electrophoretically seperated on 12.5%SDS-polyacrylamide (SDS-PAGE) gels,The results showed that IBV-T,IBV-M 41 and IBV-H 52 had the similar profile with protein of 90KD,84KD,67KD and 43KD,Protein of 61KD with high relative content appeared in IBV-T.A virulent strain of IBV isolated from Shanghai had the similar profile with IBV-T,Immunoblot analysis was performed with antibody against IBV-T,The results suggested that the protein of 61KD might be the protective antigen of IBV. |
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| Keywords: | IBV structural polypeptide SDS-PAGE western-blot |
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