| 麦秆中腥黑粉菌冬孢子的鉴定 |
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| 引用本文： | 易建平,周国梁,周业琴,印丽萍,董英. 麦秆中腥黑粉菌冬孢子的鉴定[J]. 植物保护学报, 2009, 36(4): 319-323 |
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| 作者姓名： | 易建平 周国梁 周业琴 印丽萍 董英 |
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| 作者单位： | 1. 上海出入境检验检疫局,上海,200135
2. 江苏大学,镇江,212013 |
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| 基金项目： | 科技部公益性科研专项(2007GYJ021),国家质检总局科研项目(2009IK034) 作者简介:易建平,男,1969年生,研究员,研究方向为腥黑粉菌和细菌类检疫性有害生物的检测, email: yijp@shciq.gov.cn, Tel: 021- 38620575, Fax: 021-68546481 |
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| 摘 要： | 子鉴定为小麦印度腥黑粉病菌Tilletia indica.
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| 关 键 词： | 小麦印度腥黑粉病菌 麦秆 鉴定 |
| 收稿时间： | 2008-10-10 |
Identification of the teliospores intercepted in stem debris of wheat |
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| Yi Jianping,Zhou Guoliang,Zhou Yeqin,Yin Liping and Dong Ying. Identification of the teliospores intercepted in stem debris of wheat[J]. Acta Phytophylacica Sinica, 2009, 36(4): 319-323 |
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| Authors: | Yi Jianping Zhou Guoliang Zhou Yeqin Yin Liping Dong Ying |
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| Affiliation: | Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135, China;Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135, China;Jiangsu University, Zhenjiang 212013, Jiangsu Province, China;Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135, China;Jiangsu University, Zhenjiang 212013, Jiangsu Province, China |
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| Abstract: | To identify the teliospores of genus Tilletia intercepted from stem debris of wheat, both the nested PCR and real time PCR specific to Tilletia indica were used with the sequence analysis of the PCR products from universal and specific primers. The result showed that the teliospores in stem debris of wheat got positive reaction with T.indica-spicific primer I9/I4 in nested PCR and T.indica-spicific probe TIND in real time PCR, respectively. The sequence analysis of PCR products revealed that the sequences of ITS region and PCR product from T.indica-spicific primer I9/I4 had 100% identity with those of T.indica. Based on the morphological characteristics, nested PCR detection, real time PCR assay and sequence analysis of PCR products, the teliospores from stem debris of wheat are identified as Tilletia indica. |
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| Keywords: | Tilletia indica stem debris of wheat identification |
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