| 用NASH方法检测马铃薯纺锤块茎类病毒 |
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| 引用本文： | 白艳菊,李学湛,吕典秋,何云霞,张儒喜,朱财.用NASH方法检测马铃薯纺锤块茎类病毒[J].杂粮作物,2001,21(3):42-43. |
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| 作者姓名： | 白艳菊 李学湛 吕典秋 何云霞 张儒喜 朱财 |
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| 作者单位： | 黑龙江省农业科学院生物技术中心, |
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| 摘 要： | 利用荧光素标记制备马铃薯纺缍块茎类病毒（PSTVd)特异性探针，采用核酸斑点杂交（NASH）技术对大田马铃薯和马铃薯试管苗进行检测，经放射自显影表达检测结果，结果清晰，重复性好，该方法可检测类病毒最低为0．33pg，灵敏性很高。
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| 关 键 词： | 核酸斑点杂交 马铃薯纺锤块茎类病毒 分子探针 鉴定 马铃薯 |
| 文章编号： | 1003-4803(2001)03-0042-02 |
| 修稿时间： | 2000年12月14 |
Detecting Potato Spindle Tuber Viroid by Nucleic Acid Spot Hybridization |
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| BAI Yan-Ju,LI Xue-Zhan,LU Dian-Qiu,HE Yun-Xia,ZHANG Ru-xi,ZHU Cai.Detecting Potato Spindle Tuber Viroid by Nucleic Acid Spot Hybridization[J].Rain Fed Crops,2001,21(3):42-43. |
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| Authors: | BAI Yan-Ju LI Xue-Zhan LU Dian-Qiu HE Yun-Xia ZHANG Ru-xi ZHU Cai |
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| Abstract: | The specific probe of PSTVd was prepared by using fluorescein marker. Using nucleic acid spot hybridization method to detect PSTVd in field potato and at tube tissue. The result is empressed by explose. The result is clearly and repeatable. This method can detect PSTVd sensitively at least 0.33 pg. |
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| Keywords: | nucleic acid spot hybridization potato spindle tuber viroid molecular probe |
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