L-天冬氨酸纳米钙促进油菜生长的机理机制

为揭示外源L-天冬氨酸纳米钙Ca (L-asp)-NPs]对作物生长的影响,以油菜为供试材料,通过水培试验,研究了不同浓度(0、25、50、75、100 mg·L^-1)的Ca (L-asp)-NPs和钙离子1.18 g·L^-1 Ca (NO₃)₂·4H₂O]对植株生物量、根系形态、钙含量、叶绿素、光合参数、丙二醛和可溶性蛋白含量的影响。结果表明:较低浓度(25、50 mg·L^-1)的Ca (L-asp)-NPs对油菜生长影响不显著,较高浓度(100 mg·L^-1)的Ca (L-asp)-NPs会提高植株生物量(如株高和根长)。与不施钙相比,Ca (L-asp)-NPs施用会促进油菜根系的生长,增加叶绿素含量,提高叶片光合速率,100 mg·L^-1 Ca (L-asp)-NPs处理下油菜总根长、总表面积和根尖数分别增加了76.08%、30.78%和1 107.31%;净光合速率、胞间CO₂浓度、气孔导度和蒸腾速率则分别提高了295.31%、31.90%、1 158.03%和376.74%。另外,Ca (L-asp)-NPs的施用会降低叶片丙二醛含量,提高可溶性蛋白含量。研究表明,外源Ca (L-asp)-NPs可以作为纳米肥料为油菜补充钙营养,100 mg·L^-1 Ca (L-asp)-NPs对油菜生长的促进效果最佳,油菜长势最接近于适宜钙水平。Ca (L-asp)-NPs具有作为新型纳米钙肥料在农业生产中推广的潜力。

Mechanistic mechanism of calcium L-aspartate nanoparticles to promote the growth of rape

An experiment was carried out in a green-house at Huazhong Agricultural University to reveal the effects of exogenous Laspartate nano-calciumCa (L-asp) - NPs] on the growth of rape (Brassica napus) grown hydroponically. We applied six treatments:different concentrations of Ca (L-asp)-NPs (0, 25, 50, 75, 100 mg·L^-1) and ionic calcium1.18 g·L^-1 Ca (NO₃) 2]. The dry and fresh weight, root morphological parameters, calcium content, chlorophyll, photosynthetic parameters, MDA, and soluble protein content were and improved the photosynthetic rate of leaves. Applying <100 mg·L^-1 Ca (L-asp)-NPs, the total root length, total surface area, and apical number increased significantly by 76.08%, 30.78%, and 1 107.31%, respectively; net photosynthetic rate, intercellular CO₂ concentration, stomatal conductance, and transpiration rate also increased significantly by 295.31%, 31.90%, 1 158.03%, and 376.74%, respectively. In addition, the application of Ca (L-asp)-NPs decreased MDA levels in rape leaves, while increasing the content of soluble protein in the plants. The research shows that exogenous Ca (L-asp)-NPs can be used as a nano fertilizer to supplement calcium nutrition for rape. The optimum concentration of Ca (L-asp)-NPs is 100 mg·L^-1 as this showed the best growth potential, while the growth of rape treated with <100 mg·L^-1 Ca (L-asp)-NPs has the closest to the appropriate calcium level. Ca (L-asp)-NPs has the potential to be popularized as a new nano calcium fertilizer in agricultural production. determined. The results showed that lower concentrations of Ca (L-asp)-NPs (25, 50, 75 mg·L^-1) had no significant effect on the growth of rape but a higher concentration of Ca (L-asp) - NPs (100 mg · L^-1) increased plant biomass, including plant height and root length. Compared with NPS-0, the application of Ca (L-asp)-NPs promoted the growth of rape roots, enhanced the chlorophyll content of leaves, and improved the photosynthetic rate of leaves. Applying <100 mg·L^-1 Ca(L-asp)-NPs, the total root length, total surface area, and apical number increased significantly by 76.08%, 30.78%, and 1 107.31%, respectively; net photosynthetic rate, intercellular CO₂ concentration, stomatal conductance, and transpiration rate also increased significantly by 295.31%, 31.90%, 1 158.03%, and 376.74%, respectively. In addition, the application of Ca(L-asp)-NPs decreased MDA levels in rape leaves, while increasing the content of soluble protein in the plants. The research shows that exogenous Ca(L-asp)-NPs can be used as a nano fertilizer to supplement calcium nutrition for rape. The optimum concentration of Ca(L-asp)-NPs is 100 mg·L^-1 as this showed the best growth potential, while the growth of rape treated with <100 mg·L^-1 Ca(L-asp)-NPs has the closest to the appropriate calcium level. Ca(L-asp)-NPs has the potential to be popularized as a new nano calcium fertilizer in agricultural production.