屠宰血液废弃物分解菌的筛选和鉴定

筛选高效血液蛋白分解菌株,并进行鉴定和测定其微生物酶活,为进行屠宰血液废弃物的资源化利用提供试验材料。采用酪蛋白和哥伦比亚血琼脂平板培养基,以产生透明圈的比值大小作为判定的标准,从日喀则地区屠宰场的土壤中分离纯化出1株能高效降解蛋白质的菌株,编号为NwMCC01910045。在血琼脂平板培养基上,于37℃下培养24 h,通过福林酚法测得酶活力大小为88.69 U/mL。经生物学鉴定、生理生化测定和16S rRNA序列分析,并构建系统发育树,鉴定该菌株为吉氏库特菌(Kurthia sp.),是一株能有效降解血液蛋白的菌株。

Screening and Identification of Slaughter Blood Waste Decomposing Bacteria

The purpose of this thesis was to screen high-efficiency blood proteolytic strains,and to identify and determine their microbial enzyme activities,so as to provide experimental materials for resource utilization of slaughter blood waste.In the experiment,casein and Columbia blood agar plate medium were used,and the ratio of the transparent circle was used as the criterion.A strain that can efficiently degrade protein was isolated and purified from the soil of the slaughterhouse in Xigaze area,numbered NwMCC01910045.After culturing on a blood agar plate medium at 37℃for 24 h,the enzyme activity was 88.69 U/mL as measured by the Folin method.After biological identification,physiological and biochemical determination and 16S rRNA sequence analysis,and constructing a phylogenetic tree,the strain was identified as Kurthia sp.,a strain that can effectively degrade blood proteins.