脱氧雪腐镰刀菌烯醇免疫胶体金试剂板的研制

建立了一种快速检测脱氧雪腐镰刀菌烯醇(DON)的免疫胶体金检测方法.利用柠檬酸三钠还原法制备直径40 nm的胶体金溶液,利用胶体金标记抗DON单克隆抗体制备胶体金免疫复合物,将DON-BSA和羊抗鼠IgG分别固定在NC膜的检测线和质控线上,依次将样品垫、胶体金结合垫、NC膜和吸水垫粘贴到PVC膜上,组装成试剂板.该试剂板灵敏度可达到500 μg/kg,15 min内即可完成检测；特异性好,与其他霉菌毒素无交叉反应；稳定性、准确性均较好.该胶体金免疫层析试剂板使用方便、快速,且不需要任何仪器设备辅助,可开发作为一种大批量检测脱氧雪腐镰刀菌烯醇样本的筛选手段.

Development of A Colloidal Gold Immunochromatographic Rapid-test Strip for Detection of Deoxynivalenol

A colloidal gold immunochromatographic test kit for quickly detecting deoxynivalenol was established. Preparing the colloidal gold particles of 40 nm in diameter using a method of citrate trisodium, labeling anti-DON mAb with colloidal gold particles, respectively immobilizing DON-BSA and goat-anti-mouse IgG on test-line and control-line of NC membrane, successively pasting sample pad, colloidal gold combined pad, NC membrane and absorbent pad on the PVC membrane, and assembling the test kit. Results showed that the colloidal gold immuno-complex was stable with a strong specificity and had no cross-reaction with other mycotoxins. Testing results can be obtained within 15 minutes. The colloidal gold immunochromatographic test kit was convenient in use with fast testing speed and accurate result. The testing result can be read easily without any assistance of special apparatus and instruments. and it could be developed as a batch screening method for detection of DON.