漆籽油纳米乳液的制备及其抗氧化、防紫外辐射活性研究

以漆籽油为原料,酪蛋白酸钠为乳化剂,采用高压均质法制备漆籽油纳米乳液,通过单因素试验优化制备工艺条件,考察漆籽油纳米乳液在不同温度条件下的贮藏稳定性,并评价其抗氧化活性,采用体外细胞法考察其抗紫外辐射活性。实验结果表明:漆籽油纳米乳液较佳制备工艺条件为乳化剂质量分数为2.5%,漆籽油质量分数为6%,均质压力为100MPa,均质4次,制备的纳米乳液平均粒径163.0 nm,Zeta电位-41.01 mV,多分散指数(PDI)为0.225;漆籽油纳米乳液具有很好的贮藏稳定性,在4、 25和60℃条件下贮藏30 d,平均粒径保持在200 nm以内;对DPPH、ABTS及羟基自由基的有效中浓度(EC₅₀)分别为24.26、 23.77和21.10mg/L,抗氧化活性优于阳性对照V_C(38.48、 43.25和40.56mg/L);其对紫外线辐射损伤的NIH 3T3细胞有显著的修复作用,漆籽油纳米乳液质量浓度大于5 mg/L时,辐照30 min,长波紫外线(UVA)损伤的NIH 3T3细胞存活率高于100%,具有很好的抗紫外辐射活性。

Preparation of Nanoemulsion of Lacquer Seed Oil and Its Anti-oxidation and Anti-UV Activity

Using lacquer seed oil as raw material,sodium caseinate as emulsifier,nanoemulsion of lacquer seed oil was prepared by high pressure homogenization,the optimum preparation conditions of nanoemulsion of lacquer seed oil were deter mined by single factor test;the storage stability of lacquer seed oil under different temperature was investigated;the antioxidant activity was evaluated;in vitro cellularmethod was used to investigate the anti-UV radiation activity of lacquer seed oil nanoemulsion.Results showed that the optimum preparation conditions of lacquer seed oil nanoemulsion were the mass fraction of emulsifier 2.5%,the mass fraction of lacquer seed oil 6%,homogeneous pressure 100 MPa,homogenization 4 times,the resulted nanoemulsion showed average particle size of 163.0 nm,Zeta potential of-41.01 mV,polydispersity index(PDI)of 0.225,and good storage stability,the average particle size could keep within 200 nm under storing for 30 d at 4,25,60℃.The median effective concentration(EC 50)values of DPPH,ABTS and hydroxyl radicals were 24.26,23.77 and 21.10 mg/L,which showed better antioxidant activity than positive control vitamin C(38.48,43.25and 40.56 mg/L).Lacquer seed oil nanoemulsions had significant repair effects on NIH 3T3 cells with ultraviolet A(UVA)radiation damage,the survival rate of UVA damaged NIH 3T3 cells was higher than 100%when the mass concentration of nanoemulsion was more than 5 mg/L,which indicated that lacquer seed oil nanoemulsion had good anti-UV radiation activity.