| 基于单克隆抗体的酶联免疫法与高效液相色谱法检测水产品中环丙沙星残留的比较 |
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| 引用本文： | 胡鲲,黄宣运,姜有声,方伟,朱泽闻,杨先乐.基于单克隆抗体的酶联免疫法与高效液相色谱法检测水产品中环丙沙星残留的比较[J].上海海洋大学学报,2010(6):805-809. |
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| 作者姓名： | 胡鲲 黄宣运 姜有声 方伟 朱泽闻 杨先乐 |
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| 作者单位： | 上海海洋大学农业部渔业动植物病原库, 上海 201306;广东省水生动物疫病预防控制中心, 广东 广州 510222;全国水产技术推广总站, 北京 100026 |
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| 基金项目： | 上海市科技兴农重点攻关项目(6660106477); 公益性行业(农业)科研专项(200803013);公益性行业(农业)科研专项(nyhyzx07-042); 现代农业产业技术体系建设专项资金(NYCYTX-49-17) |
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| 摘 要： | 为了快速、灵敏地检测禁用药物环丙沙星在水产品中的残留,建立了基于单克隆抗体的间接竞争酶联免疫法(competitive indirect ELISA,ci-ELISA),并比较了其与高效液相色谱法(High Performance LiquidChromatography,HPLC)在灵敏度、准确性和重现性等方法学参数上的差异。实验结果显示,经过浓缩、净化等前处理过程,ci-ELISA与HPLC的检测灵敏度分别可以达到10μg/kg和1μg/kg,回收率分别为79.28%~92.92%和86.27%~97.32%,相对标准偏差分别在3.21%~8.88%和2.66%~5.52%。前者经济、高效,适合作为初筛方法大批量筛选样品;后者灵敏度、重现性更好,适于在实验室条件下作为验证方法。
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| 关 键 词： | 环丙沙星 水产品 残留 间接酶联免疫法 高效液相色谱法 |
| 收稿时间： | 2010/1/21 0:00:00 |
Comparing the competitive indirect enzyme-linked immunosorbent assay based on monoclonal antibody and High Performance Liquid Chromatography methods to determinate the ciprofloxacin residues in fishery products |
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| HU Kun,HUANG Xuan-yun,JIANG You-sheng,FANG Wei,ZHU Ze-wen,YANG Xian-le.Comparing the competitive indirect enzyme-linked immunosorbent assay based on monoclonal antibody and High Performance Liquid Chromatography methods to determinate the ciprofloxacin residues in fishery products[J].Journal of Shanghai Ocean University,2010(6):805-809. |
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| Authors: | HU Kun HUANG Xuan-yun JIANG You-sheng FANG Wei ZHU Ze-wen YANG Xian-le |
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| Institution: | Aquatic Pathogen Collection Center of Ministry of Agriculture, Research Centre for Aquatic Drug Analysis and Measurements in Shanghai Ocean University, SOU Key Laboratory of Aquatic Genetic Resource Excavation and Utilization Certificated by Ministry of Education, Shanghai 201306, China;Guangdong Provincial Aquatic Animal Epidemic Disease Prevention and Control Center, Guanzhou 510222, China;The National Fishery Technical Extension Center, Beijing 100026, China |
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| Abstract: | |
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| Keywords: | ciprofloxacin fishery products residues competitive indirect ELISA High Performance Liquid Chromatography |
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