外源赤霉素调控胡椒花穗形成及增产机理

花穗数目是影响胡椒产量的关键因素之一,前人研究发现喷施赤霉素可以增加胡椒花穗数目,但是增穗机理尚不明确.为了揭示赤霉素调控植物由营养生长向生殖生长转变的机理,选用幼龄胡椒为试材,设置不同浓度赤霉素(20、40、60、100 mg/L)和赤霉素抑制剂多效唑(1500 mg/L),以清水为对照,进行喷施试验.研究测定胡椒的...

Exogenous Gibberellin Regulating Inflorescence Formation and Stimulation Yield Mechanism of Black Pepper

Inflorescence quantity is one of the key factors affecting pepper yield.Previous studies have found that gibberellin spraying can increase pepper inflorescence quantity,but the mechanism of inflorescence quantity increase is still unclear.To reveal the mechanism of gibberellin regulating the transition from vegetative growth to reproductive growth,in this study,young pepper was selected as the test material,with the treatment of spraying clear water as CK,different concentrations of gibberellin(20,40,60,100 mg/L)and gibberellin inhibitor paclobutrazol(1500 mg/L).The yield,inflorescence quantity,leaf morphology,photosynthetic physiology,carbohydrate content and enzyme activity related to sugar metabolism of pepper were measured.The results showed that when the concentration of exogenous gibberellin was 60 mg/L,the yield of pepper increased by 1.46 times compared with that of the control group,and inflorescence quantity increased by 1.26 times.The reasons for the increase in inflorescence quantity were analyzed and found that spraying 60 mg/L of gibberellin improved the pepper leaf area,promoted the pepper leaf photosynthesis,it was beneficial to the increase of the content of carbohydrate source organs.The treatment also promoted carbohydrate distribution to the starch in the source leaves,reducing the sucrose to starch ratio in the leaves.The feedback regulation of trehalose metabolism may be one of the reasons to promote carbohydrate distribution to starch,and the significant increase of starch synthase activity may be another reason to promote starch accumulation.Therefore,the application of gibberellin can moderately reduce trehalose content and increase starch synthase activity in pepper leaves and promote starch accumulation to increase inflorescence.The results of this study would provide practical theoretical guidance for the adoption of appropriate technical measures to promote inflorescence formation and obtain high yield pepper in production,and also help to expand the application field of gibberellin in agricultural production.