| 小麦抗白粉病基因Pm13的RAPD标记 |
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| 引用本文： | 王冰冰,孙宝启,李光威,孙群,解超杰.小麦抗白粉病基因Pm13的RAPD标记[J].中国农业大学学报,1998(Z3). |
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| 作者姓名： | 王冰冰 孙宝启 李光威 孙群 解超杰 |
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| 作者单位： | 北京海淀区圆明园西路2号中国农业大学(西校区);中国农业大学植物科技学院种子科学系; |
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| 基金项目： | 国家自然科学基金(39370432); 国家“九五”科技攻关资助项目 |
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| 摘 要： | 以近等基因系 R4A(含抗白粉病基因 Pm13)和中国春(CS)为材料,用 RAPD 技术从100个随机引物中筛选出2个具多态性的引物。片段 OPV09-1140只在 R4A 中出现,可能与 Pm13连锁;而片段OPR10-2790仅在 CS 中出现,应当是外源遗传物质替换或插入的部位。本文还对 RAPD 技术进行了探索,证明用不同时期植物材料的 DNA 作模板所得扩增带谱基本上是一致的。
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| 关 键 词： | 小麦 白粉病 抗性基因 RAPD |
Marking Powdery Mildew Resistance Gene Pm13 in Wheat by RAPD |
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| Wang Bingbing Sun Baoqi Li Guangwei Sun Qun Xie Chaojie.Marking Powdery Mildew Resistance Gene Pm13 in Wheat by RAPD[J].Journal of China Agricultural University,1998(Z3). |
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| Authors: | Wang Bingbing Sun Baoqi Li Guangwei Sun Qun Xie Chaojie |
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| Institution: | Wang Bingbing Sun Baoqi Li Guangwei Sun Qun Xie Chaojie (Dept.of Seed Science,CAU,Beijing 100094) |
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| Abstract: | RAPD technique was employed to acquire polymorphic DNA profiles from wheat near isogenic lines-Chinese Spring(CS)and R4A which carried powdery mildew resistance gene Pm13.100 random primers(Operon)were used and only two of them could produce polymorphisms between CS and R4A.OPV09-1140 appeared only in R4A and therefore, there would be a linkage between the gene Pm13 and OPV09-1140.While OPR10-2790 was produced only in CS,it ought to be the site where the alien chromsome segment inserted.Re- searches were al... |
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| Keywords: | wheat powdery mildew resistance gene RAPD |
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