Genetic diversity of Tainan-white maize inbred lines and prediction of single cross hybrid performance using RAPD markers |
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Authors: | GJ Shieh FS Thseng |
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Institution: | (1) Department of Agronomy, National Chung Hsing University, Taichung, 40227, Taiwan |
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Abstract: | To evaluate the genetic diversity of 13 maize inbred lines, and to determine the correlation between genetic distance and
single cross hybrid performance, we employed the randomly amplified polymorphic DNA(RAPD)- a PCR-based technique. Six of these
lines came from the Taichung population, and others derived from seven different sites. Forty different primers were used
to give a total of 646 reproducible amplification products, 547 (84.7%) of them being polymorphic. Genetic divergence was
determined using Jaccard's similarity coefficient, and a final dendrogram was constructed by UPGMA (unweighted pair-group
method with arithmetical averages) cluster analysis in the CLUSTER procedure of the SAS system. The RAPD analysis was a useful
tool in determining the extent of genetic diversity among Tainan-white maize inbred lines in the present case. Cluster analysis
showed that the 13 inbred lines could be classified into distinct heterotic groups. There was no significant linear regression
of grain dry weight heterosis value and mean performance of hybrids on genetic distance. And their coefficients of determination(R2) are small, so that predictive value is limited. The present results showed that the Jaccard's similarity coefficients based
on RAPD data cannot be used to precisely predict the F1 hybrids yield performance and heterosis value.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | genetic diversity heterosis RAPD Zea mays |
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