酶法提取葛根渣中异黄酮的研究

葛粉提取后形成的葛根渣含有活性成分异黄酮,将葛根渣的酶法预处理与乙醇抽提工艺相结合,可使异黄酮的提取率明显提高。以10 g葛根渣为原料,当纤维素酶用量为10 FPIU(以每克葛根渣计,下同),处理12 h后,总异黄酮得率可增至1.14%,为常规醇提法的1.36倍;在木聚糖酶用量为300 IU的条件下,处理6 h后,总异黄酮得率1.28%,为常规醇提法的1.52倍。纤维素酶和木聚糖酶之间存在着协同作用:每克葛根渣采用300 IU的木聚糖酶和7.5 FPIU的纤维素酶协同处理6 h后,总异黄酮得率可达1.38%,为常规醇提法的1.64倍。

Study on Enzymatic Extraction of Isoflavone from Pueraria (Pueraria lobata) Root Residue

Pueraria lobata(Willd.)Ohwi is a kind of raw material to produce starch in China.The root residue of pueraria from(pueraria-starch) manufactory still contains some isoflavone which is a kind of phytoestrogen with important physiological effects.By(pretreating) pueraria root residue with cellulase or xylanase before ethanol extraction,the total yield of isoflavone could be efficiently(increased.) The optimal cellulase dosage was 10 FPIU/g pueraria root residue and the suitable cellulase treatment time was 12 h.Total yield of isoflavone was 1.36 times as that by normal ethanol extraction.For pretreatment of pueraria root residue with xylanase,the(optimal) xylanase dosage was 300 IU/g residue for 6 h.Total yield of isoflavone was 1.52 times as that by regular ethanol extraction.It was proved that cellulase and xylanase could cooperate with each other to enhance extraction efficiency.When pueraria root residue was pretreated with both cellulase(7.5 FPIU/g residue) and xylanase(300 IU/g residue) for 6 h,total yield of isoflavone reached(138) mg/10 g pueraria root residue,which was 1.64 times as that by regular ethanol extraction.