| 透明质酸摇瓶发酵培养基的优化 |
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| 引用本文： | 黄小忠,曹志杰,张雪松,凡军民. 透明质酸摇瓶发酵培养基的优化[J]. 安徽农业科学, 2014, 0(1): 15-16,23 |
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| 作者姓名： | 黄小忠 曹志杰 张雪松 凡军民 |
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| 作者单位： | [1]南京农业大学生命科学学院,江苏南京210095 [2]江苏农林职业技术学院,江苏句容212400 |
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| 摘 要： | [目的]优化透明质酸摇瓶的发酵培养基。[方法]利用Plaekett—Btmnan设计及响应面分析法（RSM）对透明质酸产生菌摇瓶发酵培养基进行优化，确定影响透明质酸产量的关键因子。[结果]影响透明质酸产量的关键因子为葡萄糖和酵母膏，葡萄糖最佳浓度为55．78g／L，酵母膏的最佳浓度为23．43s／L；在此条件下，发酵液透明质酸的平均产量为0．219（‰。）。[结论]通过对培养基的优化，透明质酸产量得到提高。试验值与预测值基本一致。
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| 关 键 词： | 兽疫链球菌 优化 透明质酸 响应面分析 |
Optimization of Hyaluronic Acid Flask Fermentation Medium |
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| Affiliation: | HUANG Xiao-zhong et al (School of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095 ) |
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| Abstract: | [ Objective ] To optimize the flask fermentation medium for hyaluronie acid. [ Method ] By using Plaekett - Burman design and RSM, the flask fermentation medium for hyaluronic acid was optimized, the key factors for influencing the yield of hyaluronic acid were obtained. [ Result] The key influencing factors are glucose and yeast extract, the optimum concentrations of them are 55.78 and 23.43 g/L, re- spectively ; under the above condition, the average yield of fermentation liquor hyaluronic acid is 0.219 （ OD400nm ）. [ Conclusion] Through op- timization of culture medium, the yield of hyaluronic acid is improved, the test value is basically consistant with the predicted value. |
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| Keywords: | Streptococcus zooepidemicus Optimization Hyaluronie acid Response surface analysis |
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