| HPLC 法测定藏药八味獐牙菜丸中盐酸小檗碱的含量 |
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| 引用本文： | 逯雯洁 林鹏程. HPLC 法测定藏药八味獐牙菜丸中盐酸小檗碱的含量[J]. 安徽农业科学, 2014, 0(1): 50-51 |
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| 作者姓名： | 逯雯洁 林鹏程 |
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| 作者单位： | 青海民族大学,青海西宁810007 |
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| 摘 要： | [目的]建立藏药八味獐牙菜丸中盐酸小檗碱含量的测定方法。[方法]采用反相高效液相色谱法测定，色谱柱为依利特Hypersil C18柱（250mm×4．6mm，5μm），以乙腈-0．033mol／L磷酸二氢钾溶液（25：75，V／V）为流动相，流速1．0ml／min，检测波长为265nm。[结果]在0．1656～2．3184μg范围内，盐酸小檗碱浓度与峰面积线性关系良好，R=0．9995，平均回收率为97．30％，RSD为1．57％（n=6）。[结论]该测定方法快速、重现性好，可用于八味獐牙菜丸中盐酸小檗碱成分的含量测定。
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| 关 键 词： | HPLC 八味獐牙莱丸 盐酸小檗碱 |
Determination of the Content of Berberine Hydrochloride in Tibetan Medicine Bawei Zhangyacai Pills by HPLC |
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| Affiliation: | LU Wen-jie et al (Qinghai University for Nationalities, Xining, Qinghai 810007 ) |
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| Abstract: | [ Objective ] To establish a quantitative method to determine the contents of berberine hydrochloride in Tibetan medicine Bawei Zhangyacai Pills. [ Method ] The quantitative analysis was carried out by HPLC with a C18 column（250 mm × 4.6 ram, 5 μm）. The mobile phase was acetonitrile -0. 033 mol/L potassium dihydrogen phosphate （25：75） with a flow rate at 1.0 ml/min. The detection wavelength was set at 265 nm. [ Result] The linear ranges for berberine hydrochloride was 0. 165 6 -2. 318 4 μg （r =0. 999 5）. The average recovery was 97.30%, RSD was 1.57% （n =6）. [Conclusion] This method is simple, rapid and reliable with good reproducibility, and can be used as the determination method for berberine hydrochloride quantity in Bawei Zhangyacai Pills. |
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| Keywords: | HPLC Bawei Zhangyacai Pills Berbefine hydrochloride |
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