| 苹果轮纹病及相关病害病原菌的RAPD分析 |
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| 引用本文： | 黄春燕,刘开启.苹果轮纹病及相关病害病原菌的RAPD分析[J].植物病理学报,2001,31(2):164-169. |
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| 作者姓名： | 黄春燕 刘开启 |
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| 作者单位： | 山东农业大学植保学院植物病理学系, 泰安 271018 |
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| 摘 要： | 运用随机扩增多态性DNA (RAPD)技术对苹果轮纹病及相关病害病原菌共16个菌株进行了基因组DNA多态性分析。利用14个引物,共获得了220条RAPD标记,根据聚类分析结果,可将供试菌株分为3组,引起苹果、梨轮纹病的8个菌株为一组,引起苹果干腐病、桃流胶病以及山楂轮纹病的7个菌株为一组,轮纹大茎点属的1个菌株单独为一组。苹果轮纹病菌与干腐病菌的亲缘关系很近,但亦存在明显的差异。
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| 关 键 词： | 贝伦格葡萄座腔菌 梨生专化型 RAPD分析 轮纹病 苹果 |
| 文章编号： | 0412-0914(2001)02-0164-06 |
| 修稿时间： | 1999年11月1日 |
RAPD ANALYSIS OF THE PATHOGENIC FUNGI OF APPLE RING ROT |
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| WTBZHUANG Chun yan,LIU Kai qi WTBZ.RAPD ANALYSIS OF THE PATHOGENIC FUNGI OF APPLE RING ROT[J].Acta Phytopathologica Sinica,2001,31(2):164-169. |
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| Authors: | WTBZ]HUANG Chun yan LIU Kai qi [WTBZ] |
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| Institution: | Dept. of Plant Pathology, College of Plant Protection, Shandong Agric. Univ., Taian 271018, China |
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| Abstract: | WT5BZ] Genetic diversities of 15 isolates of Botryosphaeria berengeriana and one of Macrophoma were analyzed with random amplified polymorphic DNA (RAPD). Two hundred and twenty loci were obtained with 14 primers. The result of cluster analysis indicated that the 16 isolates could be divided into 3 groups. The first group included 8 isolates causing apple ring rot and pear ring rot. The second group included 7 isolates causing apple Botryosphaeria canker, peach gummosis and hawthorn ring rot. The third group included the isolate of Macrophoma . The genetic relationships between the pathogen of apple ring rot and that of apple Botryosphaeria canker were very close, but marked difference also existed at molecular level. |
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| Keywords: | Botryosphaeria berengeriana B berengeriana f sp piricola RAPD analysis ring rot apple |
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