| 烟草花粉植株染色体倍性苗期快速鉴定 |
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| 引用本文： | 刘仁祥,黄莺,陆永旭,蒋光华,杨双剑,雷红梅. 烟草花粉植株染色体倍性苗期快速鉴定[J]. 湖南农业大学学报(自然科学版), 2008, 34(5) |
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| 作者姓名： | 刘仁祥 黄莺 陆永旭 蒋光华 杨双剑 雷红梅 |
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| 作者单位： | 贵州大学,农学院,贵州,贵阳,550025;贵州省凤冈县烟草公司,贵州,凤冈,564200;贵州省烟草公司,贵州,贵阳,550004 |
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| 基金项目： | 贵州省"十一五"重大专项，贵州省烟草公司项目 |
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| 摘 要： | 为了提高烟草单倍体育种中花粉苗的利用效率,采用气孔保卫细胞叶绿体计数法对花粉植株染色体倍数性鉴定进行了研究.结果表明:单倍体、二倍体、多倍体的叶绿体数平均值分别为11.559,20.458,35.056个,差异极显著;通过对叶片进行失水处理,解决了幼嫩叶片不易制片的问题,结合第3,5,8,13,17叶的叶绿体数分布,将鉴定时期提到了移栽前的第5叶,得出了每株只计数1个气孔叶绿体数的一种快速、准确的计数方法--叶绿体数倍性分界法.该方法准确率高达98.5%.
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| 关 键 词： | 烟草 花粉植株 染色体倍数性 气孔保卫细胞 叶绿体数 |
Quick identification of tobacco pollen plants' chromosomes ploidy in seeding stage |
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| LIU Ren-xiang,HUANG Ying,LU Yong-xu,JIANG Guang-hua,YANG Shuang-jian,LEI Hong-mei. Quick identification of tobacco pollen plants' chromosomes ploidy in seeding stage[J]. Journal of Hunan Agricultural University, 2008, 34(5) |
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| Authors: | LIU Ren-xiang HUANG Ying LU Yong-xu JIANG Guang-hua YANG Shuang-jian LEI Hong-mei |
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| Affiliation: | LIU Ren-xiang1,HUANG Ying1,LU Yong-xu2,JIANG Guang-hua3,YANG Shuang-jian3,LEI Hong-mei1 (1. College of Agronomy,Guizhou University,Guiyang 550025,China,2. Guizhou Province Tobacco Company of Fenggang County,Fenggang,Guizhou 564200,3.Tobacco Company of Guizhou Province,Guiyang 550004,China) |
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| Abstract: | In order to increase the utilization efficiency of pollen plants in the tobacco haploid breeding,by method of counting stoma protective cell chloroplast,the identification of the chromosomes ploidy of tobacco pollen plants was conducted.The result showed that the average chloroplast number of haploid,deploid and polyploid were 11.559, 20.458,35.056 respectively,and their discrepancy was extemely prominet;the difficulty of flaking about tender leaf was solved by treatment of leaf water loss,combination with ... |
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| Keywords: | tobacco pollen plants chromosomes ploidy tobacco stoma protective cell chloroplast |
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