多重PCR技术在食品微生物检测中的应用进展

聚合酶链式反应（polymerase chain reaction，PCR）技术在微生物检测中具有快速、灵敏、操作简单等优点，而多重PCR检测还可以同时扩增多个目的基因，对高效快速检测具有重要意义，已经成为近几年来的研究热点。作者对近来多重PCR技术在食品病原微生物、食品非致病微生物及相关环境食品微生物中应用的研究现状，以及未来在食品检测中的应用展望进行了综述。

Application of Multiplex PCR in the Detection of Food Microbes

PCR technique has features of rapid, sensitive and friendly operations in the detection of microorganisms. Multiplex PCR method which can simultaneously amplificate multiple target genes is a very useful method for efficient and rapid detection of food microbes and has become a popular area in food research recently. In this paper ,the multiplex PCR applied in the detection of food-born pathogenic microorganism, non-pathogenic microorganism, and food related environmental microorganism were reviewed and its application in the future research for food microbe detections were proposed.