Isolation and characterization of 10 new microsatellite loci in the ambrosia beetle <Emphasis Type="Italic">Platypus quercivorus</Emphasis> |
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Authors: | Keiko Hamaguchi Kenryu Kato Kojiro Esaki Naoto Kamata |
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Institution: | (1) Kansai Research Center, Forestry and Forest Products Research Institute, 68 Nagaikyutaro, Momoyama, Kyoto 612-0855, Japan;(2) NIKON INSTECH Co., Ltd, 1-14-29 Ekinishi Honmachi, Kanazawa 920-0025, Japan;(3) Ishikawa-ken Forest Experiment Station, 1 Ho, Sannomiya, Hakusan 920-2114, Japan;(4) University Forest in Chichibu, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-49 Hinodamachi, Chichibu 368-0034, Japan |
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Abstract: | The ambrosia beetle, Platypus quercivorus, is an insect vector for the causal agent of Japanese oak wilt disease, Raffaelea quercivora. We have developed 10 new polymorphic microsatellite markers from Type 1, one of the genetic types of the beetle, using a
biotin-enrichment procedure. Fifty-three adult beetles were used for characterization of the markers. The number of alleles
per locus ranged from two to nine (average 4.9). The observed and expected heterozygosity ranged from 0.49 to 0.77 and from
0.44 to 0.80, respectively. None of the loci deviated significantly from Hardy–Weinberg equilibrium. No linkage disequilibrium
was detected in any pairwise comparisons of loci. Most of the markers successfully amplified in other genetic types of the
beetle. These markers could become useful tools for population genetic studies and for parentage and sibship analysis for
this beetle. |
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