Micropropagation of Cryptanthus with leaf explants with attached intercalary meristems excised from greenhouse stock plants |
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Authors: | Yong Cheong Koh Fred T Davies Jr |
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Institution: | Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843-2133, USA |
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Abstract: | By using the leaves with attached intercalary meristems from greenhouse grown stock plants, five cultivars of Cryptanthus were cultured on modified MS media with 4.5 μM NAA and IBA and 3 μM BA to induce adventitious shoot formation from callus tissue. Contamination was 17–21% for explants taken from stock plants which were sprayed weekly with Agribrom and 27–75% for those taken from stock plants which were not treated. More than 99% true to type plantlets were obtained from non-chimeric plants. Green and albino plantlets were obtained from chimeric plants. The chimeric C. ‘Coster's Favorite’ DeCoster also produced a few chimeric plantlets with intermarginal pink stripes in addition to the green and albino plantlets. Most of the non-chimeric plants took a shorter time to produce plantlets of transplantable size (8–12 mm) than the chimeric ones. Except for albino plantlets, survival rate of plantlets exceeded 95%. A minimum average of 500 rooted plantlets can be obtained in a year from a single well-callused leaf explant. The protocol in this report should speed up the mass production and introduction of desirable new cultivars and hybrids of non-chimeric Cryptanthus. |
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Keywords: | Bromeliads Cryptanthus Earthstar Intercalary leaf meristem Micropropagation Abbreviations: BA 6-benzyladenine IBA indole-3-butyric acid MS Murashige Skoog NAA naphthaleneacetic acid PPF photosynthetic photon flux |
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