| 2种三叶草杂种F1代胚性愈伤组织筛选 |
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| 引用本文： | 栾晓敏,王明玖,何丽君,李耀,李志强.2种三叶草杂种F1代胚性愈伤组织筛选[J].草地学报,2009,17(5):650-654. |
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| 作者姓名： | 栾晓敏 王明玖 何丽君 李耀 李志强 |
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| 作者单位： | 1. 内蒙古农业大学生态环境学院, 呼和浩特, 010019;2. 内蒙古农业大学农学院, 呼和浩特, 010019 |
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| 基金项目： | 国家自然科学基金，内蒙古教育基金 |
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| 摘 要： | 以高加索三叶草(Trifolium ambiguum Bieb.)与白三叶(Trifolium.repens L.)杂交后胚离体培养产生的F1无菌苗的茎段为外植体,筛选诱导愈伤组织的最适培养基,将F1茎段产生的愈伤组织经过继代后转移到分化培养基中,研究不同浓度激素组合对体细胞胚胎产生的影响。结果表明:MS+6-BA+NAA是诱导F1茎段愈伤组织的适宜培养基;MS+2,4-D+6-BA+NAA+KT+CH为F1茎段愈伤组织的适宜分化培养基;对分化后的胚性愈伤进行组织学观察,可见其表面形成许多瘤状突起,即胚性细胞团,它们可以继续发育形成体细胞胚,为快速得到数量较多的杂种F1再生苗奠定基础。
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| 关 键 词： | 高加索三叶草 白三叶 离体胚培养 杂种F1 胚性愈伤组织 |
| 收稿时间： | 2009-03-24 |
Screening of Embryogenic Callus from Hybrid F1 of Caucasian Clover×White Clover |
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| LUAN Xiao-min,WANG Ming-jiu,HE Li-jun,LI-Yao,LI Zhi-qiang.Screening of Embryogenic Callus from Hybrid F1 of Caucasian Clover×White Clover[J].Acta Agrestia Sinica,2009,17(5):650-654. |
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| Authors: | LUAN Xiao-min WANG Ming-jiu HE Li-jun LI-Yao LI Zhi-qiang |
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| Institution: | 1. College of Ecology and Environmental Science, Inner Mongolia Agriculture University, Huhhot, Inner Mongolia Autonomous Region 010019, China;2. College of Agronomy, Inner Mongolia Agriculture University, Huhhot, Inner Mongolia Autonomous Region 010019, China |
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| Abstract: | The appropriate culture medium of callus inducement was screened using the adventitious shoots as explant from the stems of aseptic seedling of F1 crossed between the embryos of Caucasian clover(Trifolium ambiguum Bieb.) and white clover(Trifolium.repens L.) by in vitro culture.The callus from stems of F1 after successive transfer culture was transferred into differentiation medium and the effect of hormone combinations with different concentrations on the somatic embryogenesis was studied.The results show ... |
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| Keywords: | Caucasian clover White clover Embryo culture in vitro Hybrid F1 Embryogenic callus |
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