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| 东北枸杞多糖分离纯化及鉴定 | |
| 引用本文： | 常建红,刘延吉,田晓艳,王玉新,王晓东. 东北枸杞多糖分离纯化及鉴定[J]. 沈阳农业大学学报, 2009, 40(5) |
| 作者姓名： | 常建红 刘延吉 田晓艳 王玉新 王晓东 |
| 作者单位： | 1. 沈阳农业大学,生物科技学院,沈阳,110161 2. 辽宁石油化工大学,环境与生物工程学院,辽宁,抚顺,113001 3. 辽宁省分析科学研究院,沈阳,110015 |
| 摘 要： | 研究了东北枸杞多糖的提取、分离纯化及其组分和结构的鉴定,结果表明:枸杞多糖水提的最佳条件为提取温度100℃,pH值11,料液比1:20,浸提2h;通过DEAE-SephadexA-50柱层析得到纯度较高的LBP-4,薄层分析LBP-4主要是由鼠李糖、木糖、葡萄糖、甘露糖和半乳糖醛酸组成.红外光谱和刚果红实验分析LBP-4具有糖类的特征吸收峰,是多种单糖通过α糖苷键及β糖苷键连接组成的杂多糖,不具有三股螺旋结构. |
| 关 键 词： | 东北枸杞 多糖 分离 纯化 鉴定 |
Isolation,Purification and Identification of Lycium barbarum Polysaccharide in Northeast Area |
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| CHANG Jian-hong,LIU Yan-ji,TIAN Xiao-yan,WANG Yu-xin,WANG Xiao-dong. Isolation,Purification and Identification of Lycium barbarum Polysaccharide in Northeast Area[J]. Journal of Shenyang Aricultural University, 2009, 40(5) | |
| Authors: | CHANG Jian-hong LIU Yan-ji TIAN Xiao-yan WANG Yu-xin WANG Xiao-dong |
| Abstract: | Isolation, purification and identification of Lycium barbarum polysaccharide in northeasarea were studied in this paper.The result showed that the optimum extraction technology of LBP was the extraction temperature 100℃,pH=11, ratio of material to water 1:20, the extraction time 2h. DEAE-SephadexA-50 purification could obtain high purity LBP-4. The analysis of TLC indicated that LBP-4 was mainly made up of Xyl., Man., Glu. and galactose acid. IR spectrum and Congo red analysis showed that LBP-4 possessed the characteristics of carbohydrate absorption peak, which is a eomplex polysaccharides composed of sugars linking through ot-glucoside lindage and β glucoside without three spiral structure. |
| Keywords: | Lycium barbarum in northeast polysaecharide Isolation purification identification |
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