Urinary excretion of theobromine in horses given contaminated pelleted food |
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Authors: | F T Delbeke M Debackere |
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Institution: | (1) Laboratorium voor Farmacologie en Toxicologie der Huisdieren, Faculteit Diergeneeskunde, Rijksuniversiteit Gent, Casinoplein 24, B-9000 Gent, Belgium |
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Abstract: | A high pressure liquid chromatographic (HPLC) method for measuring the theobromine content in cocoa husks, pelleted food and horse urine is described. Starting with 2 ml of urine, concentrations of 500 ng/ml could easily be detected. When feed containing 38.4 mg of theobromine was given twice daily to horses for 21/2 days, two days were needed after the last intake before the theobromine concentrations fell below the threshold value of 2 g/ml. The time at which the peak excretion rate occurred varied from 2 to 12 h after the last administration, while the excretion rate seemed to be dependent on the urinary flow. Theobromine could not be detected in plasma after administration in this way. |
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Keywords: | cocoa husk doping analysis horse pelleted food plasma theobromine urine |
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