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花椰菜菌核病抗性鉴定方法比较及抗病种质资源筛选
引用本文:孙溶溶,彭真,程琳,邵泰良,卢钢.花椰菜菌核病抗性鉴定方法比较及抗病种质资源筛选[J].浙江大学学报(农业与生命科学版),2011,37(6).
作者姓名:孙溶溶  彭真  程琳  邵泰良  卢钢
作者单位:1. 浙江大学农业与生物技术学院蔬菜研究所,浙江杭州,310058
2. 浙江神良种业有限公司,浙江温州,325011
基金项目:浙江省科技厅重大科技专项国际科技合作资助项目
摘    要:采用已报道的3种十字花科作物菌核病抗病性鉴定方法,在同一条件下,对5个已知抗性的花椰菜品种进行鉴定比较.结果表明:结球期琼脂块叶腋接种法、营养生长期琼脂块活体叶片法与大田接种鉴定的相关系数分别达到0.885 9和0.926 6,可有效反映抗、感品种的差异,可用于花椰菜菌核病抗性的快速鉴定;但营养生长期琼脂块离体叶片法用于抗性鉴定的效果不好;根据抗性鉴定方法的比较结果,选用结球期琼脂块叶腋接种法,以‘农乐45天’为对照,对21份花椰菜材料进行抗性鉴定,筛选获得了‘神良金花’和‘长胜65天’2份高抗材料.

关 键 词:花椰菜  菌核病  抗病性  鉴定方法  种质资源

Comparison of identification methods for resistance to Sclerotinia sclerotiorum and screening of resistant materials in cauliflower.
SUN Rong-rong,PENG Zhen,CHENG Lin,SHAO Tai-liang,LU Gang.Comparison of identification methods for resistance to Sclerotinia sclerotiorum and screening of resistant materials in cauliflower.[J].Journal of Zhejiang University(Agriculture & Life Sciences),2011,37(6).
Authors:SUN Rong-rong  PENG Zhen  CHENG Lin  SHAO Tai-liang  LU Gang
Institution:SUN Rong-rong1,PENG Zhen1,CHENG Lin1,SHAO Tai-liang2,LU Gang1 (1.Institute of Vegetable Science,College of Agriculture & Biotechnology,Zhejiang University,Hangzhou 310058,China,2.Zhejiang Sky Good Seed Co.Ltd.,Wenzhou,Zhejiang 325011,China)
Abstract:Three identification methods for the resistance to Sclerotinia sclerotiorum previously-reported in Brassicaceae crops were compared using 5 cauliflower varieties under the same condition to develop the effective screening technique in cauliflower.The results showed that the correlation between inoculating mycelium agar disk to leaf axil at seedling stage,or inoculating mycelium agar disk to live leaf at heading stage and field inoculation were highly significant(r=0.8859 and 0.9266,respectively),so these tw...
Keywords:Brassica oleracea L  var  botrytis L    Sclerotinia sclerotiorum  disease resistance  identification method  germplasm resource  
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