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小麦单倍体幼胚离体培养研究
引用本文:蔡黎明.小麦单倍体幼胚离体培养研究[J].中国农学通报,2006,22(7):313-315.
作者姓名:蔡黎明
作者单位:陕西省农业展览馆,陕西,西安,710016
摘    要:小麦单倍体幼胚由于胚乳发育异常不能提供营养物质,因此幼胚必须经过离体培养才能正常生长,而适宜的培养基是离体培养的关键。将小麦幼胚接种在8种培养基上,幼苗平均产生率分别为:30.95%、40.20%、42.20%、47.75%、45.00%、63.35%、50.00%和36.65%,结果表明1/2MS 0.2mg/LI-AA 0.2mg/L6-BAP 2%蔗糖 7.5g/L琼脂pH=5.8培养效果最好。

关 键 词:小麦  单倍体  培养基
收稿时间:2006-02-17
修稿时间:2006-02-172006-02-23

Wheat study of haploid young embryo hibemacula incubate
Cai Liming.Wheat study of haploid young embryo hibemacula incubate[J].Chinese Agricultural Science Bulletin,2006,22(7):313-315.
Authors:Cai Liming
Abstract:Since albumen of wheat young embryo cannot supply nourishment, young embryo must grow normally through in vitro culture, but the key of in vitro culture is comfort medium. We inoculate young embryo of wheat in eight medium, the frequency of seedling production follow in: 30.95%, 40.20%, 42.20%, 47.75%, 45.00%, 63.35%?50.00% and 36.65%,The results showed that the result of culture effects about pH=5.8 medium of 1/2MS, 0.2%(IAA), 0.2%(6BAP), 2% sucrose and 7.5% agar is best.
Keywords:Wheat  Haploid  Medium
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