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臭氧杀灭循环营养液中三种土传病原菌的试验
引用本文:宋卫堂,孙广明,刘芬,周立刚,王凤丽.臭氧杀灭循环营养液中三种土传病原菌的试验[J].农业工程学报,2007,23(6):189-193.
作者姓名:宋卫堂  孙广明  刘芬  周立刚  王凤丽
作者单位:1. 中国农业大学农业部设施农业生物环境工程重点开放实验室,北京,100083;中国农业大学理学院,北京,100094
2. 天津市水产研究所,天津,300221
3. 中国农业大学农学与生物技术学院,北京,100094
4. 河南省漯河市郾城区沙北办事处,漯河,462000
基金项目:国家科技攻关项目;北京市教育委员会都市农业学科群建设项目
摘    要:为给臭氧应用于循环营养液消毒提供指导,该文研究了营养液中臭氧浓度的上升、衰减,臭氧杀灭营养液中3种植物病原菌所需的残余臭氧浓度、接触时间和残留臭氧对黄瓜根系的伤害。结果表明:高浓度臭氧气体在营养液中形成的臭氧平衡浓度高,达到平衡浓度所需的时间短;低浓度臭氧气体形成的臭氧平衡浓度低,达到平衡浓度需要的时间长。当营养液中残余臭氧浓度为0.6 mg/L,接触时间5 min时,臭氧对103 cfu/mL浓度黄瓜枯萎病、番茄枯萎病和106 cfu/mL浓度十字花科软腐病3

关 键 词:臭氧消毒  营养液  植物病原菌  接触时间  残余浓度
文章编号:1002-6819(2007)6-0189-05
收稿时间:2006/9/21 0:00:00
修稿时间:2006-09-212006-11-22

Ozone disinfection of three soilborne pathogens in nutrient solution
Song Weitang,Sun Guangming,Liu Fen,Zhou Ligang and Wang Fengli.Ozone disinfection of three soilborne pathogens in nutrient solution[J].Transactions of the Chinese Society of Agricultural Engineering,2007,23(6):189-193.
Authors:Song Weitang  Sun Guangming  Liu Fen  Zhou Ligang and Wang Fengli
Institution:Key Laboratory in Bioenvironmental Engineering of Ministry of Agriculture, China Agricultural University, Beijing 100083, China;College of Science, China Agricultural University, Beijing 100094, China;Institute for Aquaculture of Tianjin, Tianjin 300221, China;College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China;College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China;Shabei Office of Yancheng District of Luohe City, Luohe 462000, China
Abstract:Experiments were conducted to determine the rising and attenuation performance of ozone concentration in nutrient solution, and the relationship between 99.99% sterilization rate of three plant pathogens and ozone concentration and the contact time. Experimental results showed that the higher the concentration of ozone air was, the higher the equation concentration of ozone in water was, and the shorter time was needed. Otherwise the lower the concentration of ozone air was, the lower the equation concentration of ozone in water was and the longer time was needed. When the ozone concentration was 0. 24;0.48 mg/L and contact time was 2 rain, the sterilization rates of the cucumber and tomato wilt pathogens, the mustard family soft rot pathogens were 99%, 99%, 99.3 %, respectively. When the ozone concentration was 0.6 mg/L and contact time was 5 rain, the three pathogens, i.e. , the cucumber and tomato wilt pathogens with initial concentration of 10^3 cfu/mL, the mustard family soft rot pathogens with initial concentration of 10^6 cfu/mL, in nutrient solution were totally killed. It was demonstrated that remaining ozone in nutrient solution was harmful to the cucumber root which had germinated for 72 h. After the roots were treated by 0.54;0. 60 mg/L and 0. 64;0.72 mg/Lozone for 30 rain, the injury rates were 7.7 %, 22.2 %, respectively. The results indicated that ozone could kill the pathogens of epiphyte and bacteria in the nutrient solution effectively, but the nutrient solution could not be applied to irrigate plants immediately after being treated by ozone.
Keywords:ozone disinfection  nutrient solution  plant pathogen  contact time  concentration
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