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| DREB1B基因在转基因小麦后代的稳定表达 | |
| 引用本文： | 荣红颖,张立全,杨凤萍,陈绪清,张晓东,郭新梅.DREB1B基因在转基因小麦后代的稳定表达[J].分子植物育种,2009,7(3). |
| 作者姓名： | 荣红颖 张立全 杨凤萍 陈绪清 张晓东 郭新梅 |
| 作者单位： | 1. 北京市农林科学院,北京农业生物技术研究中心,北京,100097;首都师范大学生命科学学院,北京,100037 2. 北京市农林科学院,北京农业生物技术研究中心,北京,100097 3. 青岛农业大学生命科学学院,青岛,266109 |
| 基金项目： | 北京市重大转基因育种平台项目，国家转基因生物新品种培育重大专项课题 |
| 摘 要： | 本研究对基因枪导入了pBAC128F/R质粒(含有玉米Adh1内含子1的CaMV35S启动子驱动的bar基因作为选择标记,以来自拟南芥菜逆境诱导表达类型--亲水蛋白rd29b基因的启动子驱动脱水应答转录因子DREBIB基因的逆境诱导表达类型质粒)的T4代转基因小麦进行了稳定表达研究.PCR、PCR-Southem和Southern杂交分析表明,外源转录因子DREBlB基因已稳定整合到转基因株系的基因组中.半定量RT-PCR结果表明,部分转基因株系的DREB1B基因的相对表达量有所增强.叶片除草剂抗性检测结果显示有8个转基因株系可抗到150 mg/L.叶片脯氨酸含量测定结果表明,有4个株系(编号为1,18,30和76)的脯氨酸含量提高幅度较大,同一株系,干旱与未干旱处理相比,脯氨酸含量提高了3～5倍.干旱处理后的转基因植株与非转基因植株相比,脯氨酸含量高2～3倍.在干旱条件下,T4代田间小区产量统计数据分析结果表明,有4个转基因株系(编号为30,51,70和76)的产量与非转基因植株相比有显著增加.研究表明,利用逆境诱导型rd29b基因的启动子来增强外源DREB1B基因的表达,能显著改良小麦的抗旱性. |
| 关 键 词： | 小麦 转基因 SEWB1B基因 脯氨酸 抗旱性 |
Stable Expression of Exogenous DREB1B in Transgenic Wheat Offspring |
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| Rong Hongying,Zhang Liquan,Yang Fengping,Chen Xuqing,Zhang Xiaodong,Guo Xinmei.Stable Expression of Exogenous DREB1B in Transgenic Wheat Offspring[J].Molecular Plant Breeding,2009,7(3). | |
| Authors: | Rong Hongying Zhang Liquan Yang Fengping Chen Xuqing Zhang Xiaodong Guo Xinmei |
| Institution: | Rong Hongying 1,2 Zhang Liquan 1 Yang Fengping 1 Chen Xuqing 1 Zhang Xiaodong 1 Guo Xinmei 3 1 Beijing Agro-Biotech Research Center,Beijing Academy of Agricultural , Forestry Science,Beijing,100097,2 College of Life Science,Capital Normal University,100037,3 College of Life Science,Qingdao Agricultural University,Qingdao,266109 |
| Abstract: | Transgenic wheat offspring, T4 generation, with plasmid pBAC128F/R (7.0 kb), which contain rd29b (induced by drought stress) promoter driven DREB1B gene, and CaMV35S promoter plus maize (Zea mays L.) Adh1 first intron driven bar gene were studied on expression stability. Analysis of PCR, PCR-Southern and Southern blot for transgenic lines of T4 generation proved that exogenous DREB1B has been integrated into genome. And semi-quantitative RT-PCR analysis indicated that DREB1B was well expressed in part of li... |
| Keywords: | Wheat Transgene DREB1B gene Proline Drought tolerance |
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