| 野茼蒿变叶病病原物的分子鉴定 |
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| 引用本文： | 车海彦,郑文虎,符瑞益,温衍生,罗大全. 野茼蒿变叶病病原物的分子鉴定[J]. 热带作物学报, 2011, 32(2): 289-292 |
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| 作者姓名： | 车海彦 郑文虎 符瑞益 温衍生 罗大全 |
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| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,海南儋州571737;农业部热带农林有害生物入侵监测与控制重点开放实验室,海南儋州571737;海南省热带农业有害生物监测与控制重点实验室,海南儋州571737
2. 中国热带农业科学院环境与植物保护研究所,海南儋州571737;海南大学环境与植物保护学院,海南儋州571737 |
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| 基金项目： | 海南省自然科学基金项目(No.310069); 中央级科研院所基本科研业务费专项(No.2011hzs1J007) |
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| 摘 要： | 利用植原体16S rDNA通用引物R16mF2/R16mR1对海南表现变叶症状的野茼蒿样品总DNA进行PCR扩增,获得约1.4 kb的特异片段,并对扩增产物进行核苷酸序列测定。通过BLAST程序比较、系统进化树构建及iPhy Classifier分析表明,海南发生的野茼蒿变叶植原体在分类上属于花生丛枝植原体组中的A亚组,即16Sr II-A亚组。这是我国首次在野茼蒿上发现植原体病害。
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| 关 键 词： | 野茼蒿变叶病 植原体 16S rDNA 分子鉴定 |
Molecular Identification of the Pathogeny of Gynura crepidioides Phyllody Disease in Hainan |
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| CHE Haiyan,ZHENG Wenhu,FU Ruiyi,WEN Yansheng and LUO Daquan. Molecular Identification of the Pathogeny of Gynura crepidioides Phyllody Disease in Hainan[J]. Chinese Journal of Tropical Crops, 2011, 32(2): 289-292 |
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| Authors: | CHE Haiyan ZHENG Wenhu FU Ruiyi WEN Yansheng LUO Daquan |
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| Affiliation: | CHE Haiyan1,2,3,ZHENG Wenhu1,4,FU Ruiyi1,WEN Yansheng1,LUO Daquan1,3 1 Environment and Plant Protection Institute,CATAS,Danzhou,Hainan 571737,China 2 Key Laboratory of Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests,Ministry of Agriculture,China 3 Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,China 4 College of Environment and Plant Protection,Hainan University,China |
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| Abstract: | A PCR product(about 1.4 kb) was amplified by employing phytoplasma universal 16S rRNA gene primers(R16mF2/R16mR1)and total DNA sample extracted from diseased Gynura crepidioides phyllody as the amplified template.Then the amplified fragments were cloned and sequenced.The results of sequences comparison of Blast program in GenBank,construction of phylogenetic tree and analysis of iPhyClassifier indicated that the phytoplasma caused Gynura crepidioides phyllody disease in Hainan belonged to Peanut witches'-br... |
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| Keywords: | Gynura crepidioides phyllody disease Phytoplasma 16S rDNA Molecular identification |
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