荧光蛋白在植物活细胞液泡成像中的应用研究进展

高等植物细胞中,液泡在各种细胞信号转导事件和形态建成中起着重要的作用。这一细胞内部结构在分裂和分化期间其功能和形状不断地变化着。为分析这些连续的变化,绿色荧光蛋白(green fluorescent protein,GFP)及其他荧光蛋白活体标记技术普遍用来跟踪特异蛋白的定位及变动。为使液泡可视成像,有几种途径可用来选择适当的荧光蛋白融合伴侣,如液泡膜内在蛋白和构造蛋白相关蛋白等就非常适合应用于液泡成像。此外,三维重建法在定位细胞内广为分布的细胞器时也不可或缺。同时,等值面表面模化过程对液泡膜成像也非常有用。本文综述液泡的结构、种类和功能,并概括各种融合的绿色荧光蛋白在植物活细胞液泡成像中的应用及其三维成像技术的研究进展。

Recent Progress in Living Cell Imaging of Plant Vacuole Using Fluorescent-protein Transgenic Lines and Three-dimensional Imaging

In high plant cells,vacuoles play important roles in a variety of cellular events,including cell division,morphogenesis,and signal transduction.These intracellular structures undergo dynamic changes in their shapes and functions during cell division and differentiation,and to analyze these sequential structural changes,the vital labeling technique,using the green-fluorescent protein or other fluorescent proteins,has commonly been used to follow the localization and translocation of specific proteins.To visu...