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| 毛细管电泳法测定槟榔多酚的组分 | |
| 引用本文： | 祁静,唐敏敏,陈卫军,黄玉林,郑亚军,赵松林.毛细管电泳法测定槟榔多酚的组分[J].热带作物学报,2011,32(2):339-344. |
| 作者姓名： | 祁静 唐敏敏 陈卫军 黄玉林 郑亚军 赵松林 |
| 作者单位： | 1. 海南大学食品学院,海南海口570228;中国热带农业科学院椰子研究所,海南文昌571339 2. 中国热带农业科学院椰子研究所,海南文昌,571339 3. 中国热带农业科学院椰子研究所,海南文昌571339;海南省热带棕榈植物重点实验室(筹),海南文昌571339 |
| 基金项目： | 海南省重点科技项目(No.090138) |
| 摘 要： | 建立同时测定槟榔壳中6种酚类物质的高效毛细管电泳方法,研究分析不同浓度和不同pH值缓冲液对10种标准品(即:表儿茶素、儿茶素、柚皮素、芦丁、山奈素、阿魏酸、绿原酸、香豆酸、咖啡酸和没食子酸)的分离效果。结果显示,最佳电解缓冲液为0.1 mol/L、pH 9.0硼酸缓冲液,紫外检测波长为280 nm,分离电压为20 kV。该方法简便快速,能在20 min之内将10种酚类物质完全分离开,具有良好的精密度、回收率和线性关系。检测限范围为0.587 5～4.415 2μg/mL。 |
| 关 键 词： | 槟榔壳 毛细管电泳方法 多酚 |
Determination of Polyphenols Presented in Areca Husk by High Performance Capillary Electrophoresis |
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| QI Jing,TANG Minmin,CHEN Weijun,HUANG Yulin,ZHENG Yajun and ZHAO Songlin.Determination of Polyphenols Presented in Areca Husk by High Performance Capillary Electrophoresis[J].Chinese Journal of Tropical Crops,2011,32(2):339-344. | |
| Authors: | QI Jing TANG Minmin CHEN Weijun HUANG Yulin ZHENG Yajun and ZHAO Songlin |
| Institution: | QI Jing1,2,TANG Minmin2,CHEN Weijun2,3,HUANG Yulin2,ZHENG Yajun2,ZHAO Songlin2,3 1 College of Food Science,Hainan University,Haikou,Hainan 570228,China 2 Coconut Research Institute,CATAS,Wenchang,Hainan 571339,China 3 Hainan Key Laboratory for Research of Tropical Palm Plants,China |
| Abstract: | The capillary electrophoresis(CE)method was developed for the simultaneous detection of a number of major polyphenol compositions of areca husk because of its bio-properties.The components analysed were(-)-epicatechin,(+)-catechin,naringenin,rutin,keampferol,ferulic acid,chlorogenic acid,coumaric acid,caffeic acid and gallic acid.Separation was achieved using a fused capillary column with 0.1 mol/L H3BO3 as the buffer at a pH of 9.0,UV detection at 280 nm and 20 kV of voltage.Analysis was carried out after ... |
| Keywords: | Areca husk Capillary electrophoresis Polyphenols |
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