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| 香蕉棒孢霉叶斑病菌的分离与鉴定 | |
| 引用本文： | 林善海,黄思良,张 贺,漆艳香,覃丽萍,谢 玲,付 岗.香蕉棒孢霉叶斑病菌的分离与鉴定[J].热带作物学报,2011,32(1):152-157. |
| 作者姓名： | 林善海 黄思良 张 贺 漆艳香 覃丽萍 谢 玲 付 岗 |
| 作者单位： | 1. 广西大学农学院,广西南宁,530004 2. 南阳师范学院生命科学与技术学院,河南南阳,473061 3. 中国热带农业科学院环境与植物保护研究所,海南儋州,571737 4. 广西农业科学院微生物研究所,广西南宁,530007 5. 广西大学农学院,广西南宁530004;广西农业科学院微生物研究所,广西南宁530007 |
| 基金项目： | 国家香蕉产业技术体系(nycytx-33); 广西科学基金(桂科自0728071) |
| 摘 要： | 从广西南宁市采集香蕉棒孢霉叶斑病样本,用常规组织分离法获得3个菌株,经柯赫氏法则验证确定其为香蕉叶斑病的病原菌。该菌的形态特征在分离培养前后变化较大,自然病斑上的分生孢子和分生孢子梗均较短,经PDA培养基分离培养后显著变长,培养后的病原菌形态特征与前人对Corynespora cassiicola的描述一致。根据形态特征及rDNA-ITS区序列比对结果,确定该病原菌株为多主棒孢菌Corynespora cassiicola(Berk&Curt)Wei]。 |
| 关 键 词： | 多主棒孢菌 症状 分生孢子 分生孢子梗 rDNA-ITS |
Isolation and Identification of Corynespora cassiicola Causing Banana Leaf Spot Disease |
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| LIN Shanhai,HUANG Siliang,ZHANG He,QI Yanxiang,QIN Liping,XIE Ling and FU Gang.Isolation and Identification of Corynespora cassiicola Causing Banana Leaf Spot Disease[J].Chinese Journal of Tropical Crops,2011,32(1):152-157. | |
| Authors: | LIN Shanhai HUANG Siliang ZHANG He QI Yanxiang QIN Liping XIE Ling and FU Gang |
| Institution: | LIN Shanhai1,HUANG Siliang2,ZHANG He3,QI Yanxiang3,QIN Liping4,XIE Ling4,FU Gang1,4 1 College of Agriculture,Guangxi University,Nanning,Guangxi 530004,PR China 2 College of Life Sciences and Technology,Nanyang Normal University,Henan,Guangxi 473061,PR China 3 Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,PR China 4 Microbiology Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi 530007,PR China |
| Abstract: | Three isolates were obtained from banana leaf spot samples collected from Nanning,Guangxi by tissue isolation.The pathogenicity of the isolates was confirmed based on Koch's postulates.The conidia and conidiophores produced on the PDA-incubated colonies became longer than those on naturally occurred banana leaf spots.The pathogenic isolate CATAS100 was identified as a member of Corynespora cassiicola based on its morphological characters on PDA medium and rDNA-ITS(ribosomal DNA internal transcribed spacer) ... |
| Keywords: | Corynespora cassiicola Symptom Conidium Conidiophore rDNA-ITS |
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