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海南龙血树的核型分析
引用本文:董美超,莫饶,唐燕琼,尹俊梅,郭娜娜,孙瑜花,王楠. 海南龙血树的核型分析[J]. 热带作物学报, 2011, 32(4): 583-586
作者姓名:董美超  莫饶  唐燕琼  尹俊梅  郭娜娜  孙瑜花  王楠
作者单位:1. 海南大学热带作物种质资源保护与开发利用教育部重点实验室,海南海口,571737
2. 中国热带农业科学研究院热带作物品种资源研究所,海南儋州,571737
基金项目:农业部热带作物种质资源利用重点开放实验室(KFKT-2009-01); 海南省自然科学基金(309013); 海南大学科研启动基金项目(kyqd1037);海南大学热带作物种质资源保护与开发利用教育部重点实验室(2010hckled-04)
摘    要:采用染色体压片技术研究了海南龙血树的染色体数目和核型。结果表明:在饱和对二氯苯和0.002 mol/L8-羟基喹啉预处理中,后者预处理2 h效果最好;海南龙血树染色体数为2n=42,核型类型为"2B"型。核型公式为:2n=2x=42=22 m+20 sm,核型不对称系数为62.67%。海南龙血树主要由中部着丝粒染色体和近中部着丝粒染色体组成,未观察到随体染色体。

关 键 词:海南龙血树  染色体数目  核型分析

Karyotype Analysis of Dracaena cambodiana Pierre ex Gagnep
DONG Meichao,MO Rao,TANG Yanqiong,YIN Junmei,GUO Nan,SUN Yuhua and WANG Nan. Karyotype Analysis of Dracaena cambodiana Pierre ex Gagnep[J]. Chinese Journal of Tropical Crops, 2011, 32(4): 583-586
Authors:DONG Meichao  MO Rao  TANG Yanqiong  YIN Junmei  GUO Nan  SUN Yuhua  WANG Nan
Affiliation:GUO Nana1,SUN Yuhua1,WANG Nan1 1 Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources,Hainan University,Ministry of Education,haikou,Hainan 570228,China 2 Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737,China
Abstract:Chromosome number and karyotype of Dracaena cambodiana Pierre ex Gagnep were investigated by chromosome spreading.Our results showed that the most suitable metaphase chromosomal phenotypes were performed when root tips treated with 0.002 mol/L 8-hydroxyquinoline.Chromosome number of Dracaena cambodiana Pierre ex Gagnep was 2n=42,with karyotypes belonging to 2B type.The karyotype formula was 2n=2x=42=22m+20sm and the index of karyotypic asymmetry was 62.67%.The diploid karyotype mainly consisted of metacenti...
Keywords:Dracaena cambodiana Pierre ex Gagnep  Chromosome number  Karyotype  
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