| 木署中miR393的RT-PCR鉴定及前体的克隆与结构分析 |
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| 引用本文： | 叶可勇,杨成龙,李瑞梅,符少萍,郭建春. 木署中miR393的RT-PCR鉴定及前体的克隆与结构分析[J]. 热带作物学报, 2011, 32(4): 627-631 |
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| 作者姓名： | 叶可勇 杨成龙 李瑞梅 符少萍 郭建春 |
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| 作者单位： | 1. 海南大学农学院，海南海口570228;中国热带农业科学院生物技术研究所,海南海口571101
2. 中国热带农业科学院生物技术研究所,海南海口,571101 |
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| 基金项目： | 中央级公益性科研院所基本科研业务“木薯抗寒转基因育种”(No.ITBBZD0721) |
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| 摘 要： | 研究木薯中表达的miRNA对深入探明木薯生长发育的调控机制具有十分重要的意义.通过RT-PCR证实木薯中有miR393的表达.采用比较基因组学的方法,从木薯基因组DNA中克隆到miR393的前体,对该miRNA前体的二级结构及与其它物种序列的同源性进行分析,结果表明,miR393前体序列在不同物种间保守性不高,但成熟的...
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| 关 键 词： | 木薯 miR393 表达 克隆 |
Identification of miR393 by RT-PCR and Cloning of miR393 Precursor in Cassava |
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| YE keyong,YANG Chenglong,LI Ruimei,FU shaoping and GUO Jianchun. Identification of miR393 by RT-PCR and Cloning of miR393 Precursor in Cassava[J]. Chinese Journal of Tropical Crops, 2011, 32(4): 627-631 |
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| Authors: | YE keyong YANG Chenglong LI Ruimei FU shaoping GUO Jianchun |
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| Affiliation: | YE keyong1,2,YANG Chenglong1,LI Ruimei2,FU shaoping2,GUO Jianchun2 1 College of Agriculture,Hainan University,Haikou 570228,China 2 Key Laboratory of Tropical Crops Biotechnology Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China |
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| Abstract: | miRNAs play important roles in the growth of cassava.In this study,the expression of miR393 was identified in cassava by RT-PCR.The miR393 precursor was cloned and sequenced from cassava through comparative genomics approach.The secondary structure was predicted.The sequence homology was compared with that from other species.Results showed that pre-miR393 was not highly conserved among different species,but matured miR393 sequence was highly conserved among different species. |
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| Keywords: | Cassava miR393 Cloning Eexpression |
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