| 中国剑麻种质资源的AFLP分析 |
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| 引用本文： | 张世清,郭朝铭,高建明,陈河龙,郑金龙,刘巧莲,易克贤.中国剑麻种质资源的AFLP分析[J].热带作物学报,2011,32(1):105-109. |
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| 作者姓名： | 张世清 郭朝铭 高建明 陈河龙 郑金龙 刘巧莲 易克贤 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南海口570208;海南大学农学院,海南海口570208
2. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南海口570208 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费项目(No.ITBBZD0828,ITBBZD0827); 农业部“948”项目(No.2010-Z6); 海南省自然科学基金项目(No.80510); 国家支撑计划项目(No.2007BAD48B06-05) |
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| 摘 要： | 利用荧光标记AFLP技术研究剑麻种质遗传多样性及亲缘关系。筛选8个多态性高、分辨力强的Pst I/Mse I引物组合对40份供试材料的基因组DNA扩增,共获得条带数785条,多态性条带数507条,扩增效率64.58%,平均每对引物扩增条带数98.13条。其中,以P-GAC/M-CAC引物的扩增效率最高,达到78.63%;其次是P-GTG/M-CTC,达到71.28%;扩增效率最低的引物是P-GTA/M-CTT,为52.94%。表明供试材料在DNA水平上,酶切位点的分布存在广泛的变异。各供试材料相似系数介于0.44～0.83,在相似系数0.51处可将40份种质划分为4大类。8组引物在40份供试材料中检测到数目不等的特异带型,这些特异带型对供试剑麻种质具有一定的鉴别价值。
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| 关 键 词： | 剑麻 AFLP 遗传多样性 亲缘关系 |
AFLP Analysis of Sisal Varieties in China |
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| ZHANG Shiqing,GUO Chaoming,GAO Jianming,CHEN Helong,ZHEN Jinlong,LIU Qiaolian and YI Kexian.AFLP Analysis of Sisal Varieties in China[J].Chinese Journal of Tropical Crops,2011,32(1):105-109. |
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| Authors: | ZHANG Shiqing GUO Chaoming GAO Jianming CHEN Helong ZHEN Jinlong LIU Qiaolian and YI Kexian |
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| Institution: | ZHANG Shiqing1,2,GUO Chaoming1,GAO Jianming1,CHEN Helong1 ZHEN Jinlong1,LIU Qiaolian1,YI Kexian1 1 Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural SciencesKey Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture Haikou,Hainan 571101,China 2 College of Agronomy,Hainan University,Haikou,Hainan 570208,China |
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| Abstract: | The genetic diversity and relationship of sisal were analyzed by fluorescent AFLP technology.AFLP fingerprinting of 40 accessions with eight pairs of Pst I/Mse I primers revealed a total number of 785 unambiguous bands including 507 polymorphic bands,and the polymorphism frequency was 64.58%.Each pair of primers produced 98.13 polymorphic bands on average.The primer P-GAC/M-CAC had the highest polymorphism frequency(78.63%),followed by the primer P-GTG/M-CTC(71.28%).The primer P-GTA/M-CTT had the least poly... |
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| Keywords: | Sisal AFLP Genetic diversity Relationship |
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