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| 银合欢AFLP分子标记体系的优化 | |
| 引用本文： | 吴成贡,邹冬梅,张景千,蒋昌顺. 银合欢AFLP分子标记体系的优化[J]. 热带作物学报, 2011, 32(4): 640-643 |
| 作者姓名： | 吴成贡 邹冬梅 张景千 蒋昌顺 |
| 作者单位： | 1. 中国热带农业科学院热带作物品种资源研究所， 海南儋州571737;海南大学农学院, 海南儋州571737 2. 中国热带农业科学院热带作物品种资源研究所,海南儋州,571737 3. 海南大学农学院,海南儋州,571737 |
| 基金项目： | 国家自然科学基金项目(30860197) |
| 摘 要： | 以银合欢幼苗为材料,采用CTAB区室法和试剂盒法2种方法提取DNA,通过对影响AFLP反应关键因子,如DNA样品酶切时间、引物等进行优化,建立了适合银合欢AFLP分子标记的反应体系,为利用AFLP标记对银合欢进行分子生物学研究及分子育种奠定基础。 |
| 关 键 词： | 银合欢 DNA AFLP |
Optimizing AFLP Molecular Marker System for Leucaena |
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| WU Chenggong,ZOU Dongmei,ZHANG Jingqia and JIANG Changshun. Optimizing AFLP Molecular Marker System for Leucaena[J]. Chinese Journal of Tropical Crops, 2011, 32(4): 640-643 | |
| Authors: | WU Chenggong ZOU Dongmei ZHANG Jingqia JIANG Changshun |
| Affiliation: | WU Chenggong1,2,ZOU Dongmei1,ZHANG Jingqian2,JIANG Changshun1 1 Tropical Crops Genetic Resources Institute,CATAS,Danzhou,Hainan 571737,China 2 College of Agronomy,Hainan University,China |
| Abstract: | DNA was extracted from the materials of Leucaena seedlings with CTAB and kit.The suitable AFLP molecular marker system was established for Leucaena diversity analysis after AFLP reaction had been optimized with DNA samples in different digestion time and primers screening.This work provided a basic knowledge on AFLP molecular marker for studying Leucaena molecular biology and molecular breeding. |
| Keywords: | Leucaena DNA AFLP |
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