|
|||||
|
|
| 一株拮抗香蕉枯萎病菌的链霉菌分离和鉴定 | |
| 引用本文： | 卢娟,夏启玉,孙建波,卢雪华,王宇光,张昕. 一株拮抗香蕉枯萎病菌的链霉菌分离和鉴定[J]. 热带作物学报, 2011, 32(2): 278-282 |
| 作者姓名： | 卢娟 夏启玉 孙建波 卢雪华 王宇光 张昕 |
| 作者单位： | 1. 海南大学农学院,海南儋州,571737 2. 中国热带农业科学院生物技术研究所,海南海口571101;农业部热带作物生物技术重点开放实验室,海南海口571101 3. 中国热带农业科学院环境与植物保护研究所,海南儋州,571737 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项(No.ITBBZX2008-2-3) |
| 摘 要： | 从海南温泉中分离到1株对香蕉枯萎病病原菌4号小种有强拮抗作用的链霉菌菌株HW1,对该菌株进行了形态特征、培养特征、生理生化特征及16S rDNA序列分析。并对该菌株和香蕉枯萎病病原菌4号小种在土壤中进行了不同温度下的共培养试验。结果表明,其在PDA培养基上,内生菌丝无横隔、不断裂;孢子圆形,簇聚在气生菌丝上。在PDA培养基上,菌落生长开始为白色,培养成熟后,气丝逐渐由白色变灰,最后因为产生孢子变为褐色,并可产黄色可溶色素。以16S rDNA序列为基础构建了菌株HW1的系统发育树,其与模式链霉菌株的16S rDNA序列的同源性为99%。初步将该菌株鉴定为Streptomyces costaricanus。HW1菌株和香蕉枯萎病病原菌4号小种在土壤中的共培养试验表明,HW1菌株在45℃温度的生长环境中,表现出对香蕉枯萎病病原菌较好的抗性。 |
| 关 键 词： | 香蕉枯萎病 链霉菌HW1 分离 鉴定 共培养 |
Isolation and Identification of a Streptomyces Strain against Banana Wilt Disease Caused by Fusarium oxysporum f. sp. cubense |
|
| LU Juan,XIA Qiyu,SUN Jianbo,LU Xuehu,WANG Yuguang and ZHANG Xin. Isolation and Identification of a Streptomyces Strain against Banana Wilt Disease Caused by Fusarium oxysporum f. sp. cubense[J]. Chinese Journal of Tropical Crops, 2011, 32(2): 278-282 | |
| Authors: | LU Juan XIA Qiyu SUN Jianbo LU Xuehu WANG Yuguang ZHANG Xin |
| Affiliation: | LU Juan1,XIA Qiyu2,SUN Jianbo2,LU Xuehua2,WANG Yuguang2,ZHANG Xin3 1 College of Agriculture,Hainan University,Danzhou,Hainan 571737,China 2 Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China 3 Environment and Plant Protection Institute,China |
| Abstract: | A Streptomyces strain named HW1 against banana wilt caused by Fusarium oxysporum f.sp.cubense race 4(FOC4)was isolated from a hot spring in Hainan Province.The morphological characteristics,cultural characteristics,physiological,biochemical characteristics and 16S rDNA sequences analysis of Streptomyces HW1 were studied.Besides,the co-culture tests in soil between HW1 and FOC4 were studied at different temperature.The results showed that,on PDA medium,the substrate mycelia of HW1 had no partition,and the sp... |
| Keywords: | Banana wilt disease Streptomyces HW1 Isolation Identification Co-culture |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《热带作物学报》浏览原始摘要信息 | |
| 点击此处可从《热带作物学报》下载全文 | |