|
|||||
|
|
| 山羊毛囊干细胞分离、培养及鉴定 | |
| 引用本文： | 史明艳,高雪,杨学义,窦忠英. 山羊毛囊干细胞分离、培养及鉴定[J]. 畜牧兽医学报, 2008, 39(12) |
| 作者姓名： | 史明艳 高雪 杨学义 窦忠英 |
| 作者单位： | 1. 洛阳师范学院生命科学系,洛阳,471022;中国农业科学院北京畜牧兽医研究所 2. 中国农业科学院北京畜牧兽医研究所 3. 洛阳师范学院生命科学系,洛阳,471022 4. 西北农林科技大学,国家干细胞工程技术研究中心陕西分中心,杨凌,712100 |
| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，教育部重大科技专项项目 |
| 摘 要： | 采用2．4U／mLDispase酶消化和机械切割法分离毛囊隆突部，经胰酶（0．5mg／mL胰酶＋0．2mg／mLEDTA）消化从山羊耳部皮肤分离得到毛囊干细胞，用自制无血清培养基培养，并用形态学观察、细胞生长曲线、克隆形成率及免疫组化染色检测，探讨毛囊干细胞体外分离培养方法及生物学特性。结果表明，所分离细胞具备毛囊干细胞特征：体积较小、表面光滑、立体感强，呈现未分化细胞特征；K19、integrin-β1以及p63免疫组化染色阳性；第3、5、7代干细胞克隆形成率分别为25．6％±2．6％、31．8％±1．9％和27．0％±3．9％，极显著高于对照组角质细胞克隆形成率（P〈0．01）。采用配制的无血清条件培养液可使山羊毛囊干细胞体外维持未分化状态并传代至19代。 |
| 关 键 词： | 山羊 毛囊干细胞 毛囊隆突部 克隆形成率 |
Isolation,Culture and Identification of Goat Hair Follicle Stem Cells |
|
| SHI Ming-yan,GAO Xue,YANG Xue-yi,DOU Zhong-ying. Isolation,Culture and Identification of Goat Hair Follicle Stem Cells[J]. Chinese Journal of Animal and Veterinary Sciences, 2008, 39(12) | |
| Authors: | SHI Ming-yan GAO Xue YANG Xue-yi DOU Zhong-ying |
| Affiliation: | SHI Ming-yan1,3,GAO Xue2,YANG Xue-yi1,DOU Zhong-ying3*(1.Life Science Department of Luoyang Normal College,Luoyang 471022,China,2.Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,3.Shaanxi Branch of National Stem Cell Engineering Center,Northwest A&F University,Yangling 712100,China) |
| Abstract: | Hair follicles were isolated from goat ear skin by 2.4 U/mL Dispase digestion and the bulges were separated with special fine needle and digested with 0.5 mg/mL trypsin plus 0.2 mg/mL EDTA.The resultant cells were cultured in self-prepared serum-free conditional medium and studied through morphological observation,cell growth curve and colony-forming efficiency(CFE),and identified by immunohistochemical staining.The results showed that the cells were slide,small-sized,ball-shped,obviously three-dimensional ... |
| Keywords: | goat hair follicle stem cells hair follicle bulge colony-forming efficiency |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |