不同基因型高山杜鹃茎段离体培养及工厂化繁育体系优化

为了优化高山杜鹃茎段离体组织培养体系及其工厂化繁育体系,对10份不同基因型高山杜鹃离体培养与植株再生差异进行了研究。结果表明,供试高山杜鹃茎段外植体在WPM培养基上均能诱导出芽、增殖和生根。在诱导萌发阶段,添加0.5～1.0 mg/L的ZT萌发效果较好,其中有7份材料在0.5 mg/L ZT时获得最佳萌发诱导。在增殖阶段,最佳增殖激素配比为ZT 0.5～0.7 mg/L+NAA 1.0 mg/L,其中6份材料在ZT 0.5 mg/L+NAA 1.0 mg/L激素配比下增殖达6～9倍。生根阶段,培养基最佳激素配比则为NAA 0.25mg/L+IBA 0.25 mg/L。并且试管外生根效果好于试管内生根,平均成活率为80.95%,而试管内平均成活率仅为57.94%。在最佳激素配比下供试所有高山杜鹃采用试管外生根成活率均在98%以上,并且试管外生根成本低,操作简便快速,适用于工厂化生产。

Optimization of in vitro Culture of Stem Segment and Factory Propagation System of Rhododendron lapponicum

To optimize the in vitro culture system and factory propagation system from stem fragments,the differences of in vitro regeneration and culture among ten genotypes of Rhododendron lapponicum were investigated.The results indicated that the explants of stem fragments from all Rhododendron lapponicum genotypes could be induced to germinate,proliferate and take root on the WPM medium.During the process of inducing adventitious buds,the germination efficiency was better by adding 0.5~1.0 mg/L zeatin,in which,seven genotypes got the best germination at 0.5 mg/L zeatin.At the multiplication stage,0.5~0.7 mg/L ZT + 1.0 mg/L NAA was the best,and the multiplication rate of six genotypes treated by ZT 0.5 mg/L + NAA 1.0 mg/L was about 6~9 times higher than that without any hormone treatment.At the rooting stage,the best hormone ratio was NAA 0.25 mg/L + IBA 0.25 mg/L,and the rooting out of tube with the survival rate of 80.95% was better than that in tube with the survival rate of 57.94%.Under the hormone treatment with the best ratio,the survival rate of all genotypes was over 98%.Meanwhile,the rooting out of tube cost lower and could be operated more conveniently,so it was suitable for factory propagation.