Functional Analysis of DHS by Virus-induced Gene Silencing in Nicotiana benthamiana

With the progress in plant genomics research, the study direction is turned into the discovery and functional analysis of important genes. But an effective method is needed to investigate the biological function of eDNA sequences in large-scale （Wang et al., 2005）. With the newly developed technique-virus-induced gene silencing （VIGS）, plants infected with virus vector carrying host-derived sequence inserts will show loss-of-function or reduced-expression mutants in the host gene. The symptoms will tell the functional information of the gene （Liu et al., 2004; Burton et al., 2000）. Normally the process of constructing a virus vector and monitoring symptoms on infected plants can be completed within a few weeks, such that VIGS provides a simple, rapid and high throughput means of analyzing the function of sequenced genes.

Functional Analysis of DHS by Virus-induced Gene Silencing in Nicotiana benthamiana

With the progress in plant genomics research, the study direction is turned into the discovery and functional analysis of important genes.