莱芜猪和杜洛克猪H-FABP表达差异和肉质性状关系研究

采用荧光定量RT-PCR法测定H-FABP基因在体重100kg左右的莱芜猪和杜洛克猪背最长肌中的表达差异,分析了H-FABP基因表达差异与肉质性状的关系,结果表明：莱芜猪的背最长肌H-FABP基因mRMA的表达水平比杜洛克猪高38.60％,认为可能与莱芜猪肌内脂肪含量极显著高于杜洛克猪有关（P<0.01）;H-FABP基因的背最长肌mRNA的表达水平与肌内脂肪、pH值成正相关,与肌纤维直径成负相关,与大理石纹和肉色的相关关系两品种不一致。其中莱芜猪和杜洛克猪H-FABP基因mRNA表达量与肌内脂肪相关系数最高,分别为0.363和0.943。对H-FABP基因的研究不仅有助于肌内脂肪的提高,而且可提高肌肉pH值、降低肌纤维直径,全面改善肌肉的肉质性状

Difference of H-FABP Expression in Laiwu Pigs and Duroc and its Relationships with Meat Quality

Difference of H-FABP mRNA transcription of Longissimus dorsi in Laiwu pigs and Duroc weighted about 100kg were detected by fluorescence quantitative RT-PCR. And its relationship with meat quality was analyzed. The result indicated that the H-FABP expression of Laiwu pigs was higher 38.60％ than it of Duroc, and perhaps it was related with the fact that IMF of Laiwu pigs were significantly higher than it of Duroc pigs（P<0.01）. The correlation between the H-FABP expression with IMF and pH were positive, with diameter of musclur fiber negative, and with marble score and meat color were not consistent in Laiwu pigs and Duroc. Thereinto, the correlation coefficients between the H-FABP expression with IMF were highest, 0.363 and 0.934, respectively. Therefore study of H-FABP can not only increase the IMF content, but increase pH and decrease diameter of musclur fiber.