| 高酶活纤维素分解菌分离筛选的研究 |
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| 引用本文： | 岳思君,李学斌,李爱华,蒙秋燕.高酶活纤维素分解菌分离筛选的研究[J].安徽农业科学,2009,37(1):11-12. |
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| 作者姓名： | 岳思君 李学斌 李爱华 蒙秋燕 |
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| 作者单位： | 宁夏大学生命科学学院,宁夏银川,750021;宁夏大学西部生态与生物资源开发联合研究中心,宁夏银川,750021 |
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| 基金项目： | 国家“863”计划项目(2005AA001330); 国家农业科技成果转化资金项目(2006GB2G300314); 宁夏大学自然基金重点项目(2004-029) |
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| 摘 要： | 目的]筛选有良好分解效果的纤维素分解菌。方法]以含菌秸秆、腐叶和玉米地土壤为材料，经富集培养后，根据水解圈直径进行纤维素分解菌初筛分离培养和复筛培养，制取粗酶液，测定CMC酶活力和滤纸酶活。结果]从各地采集的样品中共分离到6个菌株，各菌株均能在羧甲基纤维素钠培养基上较好生长，其中菌株H-1、H02、H．6生长最快，而其余菌株则生长缓慢，菌株H-2的CMC酶活和滤纸酶活分别为0．2114和0．2950IU／ml，菌株H-6的CMC酶活和滤纸酶活分别为0．2016和0．2802IU／ml，高于其他4种菌株；菌株H4的产纤维素酶能力最低，CMC酶活和滤纸酶活分别为0．1819和0．2065IU／ml。结论]H-2、H-6菌株分解纤维素的能力最强。
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| 关 键 词： | 纤维素分解菌 CMC酶活 滤纸酶活 水解圈 |
Study on Isolation and Screening for Cellulose-decomposing Bacterial with High Cellulase Activity |
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| YUE Si-jun et al.Study on Isolation and Screening for Cellulose-decomposing Bacterial with High Cellulase Activity[J].Journal of Anhui Agricultural Sciences,2009,37(1):11-12. |
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| Authors: | YUE Si-jun |
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| Institution: | YUE Si-jun et al (College of Life Science,Ningxia University,Yinchuan,Ningxia 750021) |
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| Abstract: | Objective] The aim was to screen cellulose-decomposing bacteria with good decomposition effect.Method] With straw that contained bacterium,rotten leaves and soil in maize field as materials,after the enrichment culture,the bacteria was isolated and cultured according to the diameter of hydrolyzed circle for primary and secondary screening.The crude enzyme liquid was prepared for the determination of CMCase activity and filter paper enzyme activity.Result] 6 strains were isolated from the examples in diff... |
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| Keywords: | Cellulose-decomposing microorganisms CMCase activity Filter paper enzyme activity Hydrolyzed circle |
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