应用酸性电解水联合超声波杀灭副溶血性弧菌

将酸性电解水与超声波技术相结合,探究其对副溶血性弧菌的杀灭情况,并与其他杀菌措施进行对比。采用平板计数法进行菌落计数,扫描电镜观察细菌的形态变化,蛋白质泄漏揭示细胞膜通透性差异,并结合流式细胞仪分析生物学特征的改变,分别比较了酸性电解水、超声波以及联合处理对副溶血性弧菌的杀菌效果。结果显示:酸性电解水联合超声波处理可使副溶血性弧菌的数量减少2.09 log CFU/mL,亚致死菌数量为1.80 log CFU/mL,而仅用超声波处理,细菌只减少了0.63 log CFU/mL,亚致死菌数量为0.05 log CFU/mL(P0.05)。扫描电镜结果表明,电解水联合超声波处理对副溶血性弧菌的细胞结构有明显的破坏作用,结合二喹啉甲酸法(BCA)显示其细胞内蛋白质泄漏226.596μg/mL(P0.05)。进一步的流式细胞仪分析结果显示,经联合处理后细菌细胞明显缩小,颗粒度变化增大。综上所述,相较于酸性电解水或超声波单一处理,通过菌落计数,细菌的形态变化,蛋白质泄漏与细胞生物学特征的改变可知,酸性电解水联合超声波的处理方式具有更强的杀菌效果,可作为一种新型的技术应用于水产品中副溶血性弧菌的杀灭。

Combination of acidic electrolyzed water with ultrasonic for killing Vibrio parahaemolyticus

This study evaluated the bactericidal effects of Vibrio parahaemolyticus by acidic electrolyzed water (AEW) combined with ultrasound which is the method different from the traditional sterilization method, The effects were compared by viable plate count analysis. The physiological response was studied by scanning electronic microscopy (SEM) and cell membrane permeability revealed by protein leakage. Flow cytometry analysis revealed changes in the physiological states of Vibrio parahaemolyticus after acidic electrolyzed water, ultrasound and the combination of acidic electrolyzed water combined with ultrasonic. The results revealed that AEW combined with ultrasonic showed better bacterial reduction in Vibrio parahaemolyticus of 2.09 log CFU/mL and the sublethal bacterial reduction was 1.80 log CFU/mL, When compared to the reductions of 0.63 log CFU/mL and the sublethal bacterial reduction of 0.05 log CFU/mL after the individual ultrasonic treatments. It had great impacts on the biological structure from the SEM images, the permeability of the cell membrane increased. Flow cytometry analysis showed that the cells became smaller and the change in particle size increased. In summary, acidic electrolyzed water combined with ultrasound has a stronger bactericidal effect compared with single treatment . It can be used as a new sterilization technique to reduce the risk of Vibrio parahaemolyticus and improve the quality and safety of aquatic products.