首页 | 本学科首页   官方微博 | 高级检索  
     检索      

藏鸡和隐性白羽鸡GH基因的SNP检测及其与生长性状间的关联分析
引用本文:李长春,傅田,强巴央宗,唐晓惠,朱猛进,樊斌,熊统安,李奎,刘榜.藏鸡和隐性白羽鸡GH基因的SNP检测及其与生长性状间的关联分析[J].中国农业科学,2005,38(11):2327-2332.
作者姓名:李长春  傅田  强巴央宗  唐晓惠  朱猛进  樊斌  熊统安  李奎  刘榜
作者单位:1. 华中农业大学动物科技学院动物分子生物学与育种实验室,武汉,430070
2. 西藏大学西藏农牧学院牧医系,林芝,860000
基金项目:引进国际农业科学技术项目(948)(2001-361)和国家“863”项目(2001AA222241)资助
摘    要: 利用改良等位基因特异性PCR(Modified Allele Specific PCR, M-ASP)结合PCR-RFLP以及直接测序方法对藏鸡和隐性白羽鸡生长激素(Growth Hormone, GH)基因内含子4 (Intron4)的一个位点进行了SNP检测,并进行了该基因与藏鸡生长性状的关联分析。检测结果显示,GH基因Intron4的这个位点同时具有M-ASP和SacI-RFLP多态。测序结果表明,该位点发生了C→G的碱基突变,产生了CC、CG和GG三种基因型。两个等位基因和三种基因型在两鸡种中的分布基本一致。其中,基因型CC和等位基因C的频率最高。χ2检验结果表明,基因型频率或者等位基因频率在两鸡种之间没有显著差异(P>0.05),而分别在两鸡种内部却存在显著(P<0.05)或极显著(P<0.01)差异。方差分析结果表明,基因型与藏鸡的2周龄体重等12个生长性状有显著(P<0.05)或极显著(P<0.01)关联;基因型CC与藏鸡的7周龄体重存在显著关联(P<0.05)。这暗示了GH基因是影响藏鸡生长的主效基因或者它与主效基因相关,而该位点的碱基突变可以作为筛选藏鸡高的7周龄体重的分子标记。

关 键 词:藏鸡和隐性白羽鸡  SNP  M-ASP  GH基因Intron4  生长性状  关联分析
收稿时间:11 22 2004 12:00AM
修稿时间:2004-11-22

SNP Detection in Tibetan Chicken and Recessive Chicken GH Gene and Its Associations with Growth Traits
LI Chang-chun,FU Tian,QIANG BA Yang-zong,TANG Xiao-hui,ZHU Meng-jin,FAN Bin,XIONG Tong-an,LI Kui,LIU Bang.SNP Detection in Tibetan Chicken and Recessive Chicken GH Gene and Its Associations with Growth Traits[J].Scientia Agricultura Sinica,2005,38(11):2327-2332.
Authors:LI Chang-chun  FU Tian  QIANG BA Yang-zong  TANG Xiao-hui  ZHU Meng-jin  FAN Bin  XIONG Tong-an  LI Kui  LIU Bang
Institution:1 Laboratory of Molecular Biology andAnimalBreeding, School of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070; 2Department of Animal Science, Tibet Agriculture and Animal Husbandry College, Linzhi 860000
Abstract:SNP of growth hormone (GH) gene Intron4 of Tibetan chicken and recessive white chicken were detected by using modified allele specific PCR (M-ASP), PCR-RFLP and sequencing reaction and its associations with growth traits of Tibetan chicken was done. The results indicated that there were M-ASP and SacI-RFLP polymorphisms in Intron4 of GH gene and there was a C/G mutation by DNA sequencing and resulted in 3 genotypes CC, CG and GG. The distributions of three genotypes and two alleles between two chicken breeds were very similar and the frequencies of genotype CC and allele C were highest among them. The results χ2 showed that there were not significant differences in the frequencies of genotypes or alleles between the two chicken breeds (P>0.05), however, there were significant differences within each of the two chicken breeds (P<0.05), respectively. The results of analysis of variance showed that genotypes had significant effect on twelve growth traits of Tibetan chicken at 0.05 or 0.01 levels, and there was a positive relationship between genotype CC and body weight of Tibetan chicken at the age of 7 weeks (P<0.05). It implies that GH gene is the major gene affecting growth traits of Tibetan chicken or it links with the major gene, and the mutation could be used as the molecular genetic marker to select Tibetan chicken for high body weight at the age of 7 weeks.
Keywords:Tibetan chicken and recessive white chicken  SNP  M-ASP  Intron4 of GH gene  Growth traits  Association analysis
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《中国农业科学》浏览原始摘要信息
点击此处可从《中国农业科学》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号