| 苹果Ty1-copia类逆转座子LTR10序列及其在苹果属植物中的遗传多样性分析 |
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| 引用本文： | 孙俊,房经贵,王飞,孙其宝,章镇,王昆. 苹果Ty1-copia类逆转座子LTR10序列及其在苹果属植物中的遗传多样性分析[J]. 南京农业大学学报, 2010, 33(1) |
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| 作者姓名： | 孙俊 房经贵 王飞 孙其宝 章镇 王昆 |
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| 作者单位： | 南京农业大学园艺学院,江苏,南京,210095;安徽农业大学园艺学院,安徽,合肥,230036;南京农业大学园艺学院,江苏,南京,210095;安徽省农业科学院园艺研究所,安徽,合肥,230031;中国农业科学院果树研究所,辽宁,兴城,125100 |
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| 基金项目： | 国家自然科学基金项目(30200190,30740034);;安徽农业大学稳定与引进人才科研启动专项基金项目(wd2006-8) |
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| 摘 要： | 应用改良的Pearce方法分离苹果Ty1-copia类逆转座子RNaseH-LTRs,分离到的RNaseH-LTR_(10)序列已在GenBank注册(登录号DQ534515),该序列长度为299 bp。分析结果表明其5′端为含有终止密码子的RNaseH基因,PPT(polypurinetract)之后是3′-LTR,PPT起始于终止密码子内10 bp处,3′-LTR的起始标志末端倒转重复序列(inverted repeat,IR)TG紧随其后。LTR_(10)的正链和反链均含有多个启动子的特征结构TATA box和CAAT box,α-淀粉酶启动子的保守序列及受不同胁迫条件作用的调控元件,如AuxRE、ABRE、HSE等。利用A-SAP技术研究了LTR_(10)逆转座子在苹果属8个野生种和22个栽培品种中的遗传多样性,多态性片段比例为86.5%;品种长祝较祝光少1条约400 bp的特异性扩增条带。
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| 关 键 词： | 苹果 Ty1-copia类逆转座子LTRs 调控元件 遗传多样性 |
Analysis on the sequence of Ty1-copia retrotransposon LTR10 and its genetic diversity in Malus genus |
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| SUN Jun,FANG Jing-gui,WANG Fei,SUN Qi-bao,ZHANG Zhen,WANG Kun. Analysis on the sequence of Ty1-copia retrotransposon LTR10 and its genetic diversity in Malus genus[J]. Journal of Nanjing Agricultural University, 2010, 33(1) |
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| Authors: | SUN Jun FANG Jing-gui WANG Fei SUN Qi-bao ZHANG Zhen WANG Kun |
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| Affiliation: | 1/a>;2;1*;1.College of Horticulture/a>;Nanjing Agricultural University/a>;Nanjing 210095/a>;China/a>;2.College of Horticulture/a>;Anhui Agricultural University/a>;Hefei 230036/a>;3.Horticulture Research Institute/a>;Anhui Academy of Agricultural Sciences/a>;Hefei 230031/a>;4.Research Institute of Pomology/a>;Chinese Academy of Agricultural Sciences/a>;Xingcheng 125100/a>;China |
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| Abstract: | We used the technique implemented by Pearce to isolate RNaseH-LTRs from the apple accession Gala genome.The amplified sequence RNaseH-LTR_(10) has been submitted to GenBank(Accession number:DQ534515).The length of the RNaseH-LTR _(10) was 299 bp.The RNaseH-LTR_(10) contained a recognizable RNaseH gene with stop codon at 5' end of the sequence and the characteristic polypurine tract(PPT) followed by the putative 3'-LTR region.The PPT was located at minus 10 bp downstream form the stop codon of the RNaseH gen... |
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| Keywords: | apple LTRs of Ty1-copia-like retrotransposon regulatory elements genetic polymorphism |
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