| 我国油松主要分布区种质资源遗传多样性 |
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| 引用本文： | 王磊,樊军锋,刘永红,杨培华,王孟昌. 我国油松主要分布区种质资源遗传多样性[J]. 东北林业大学学报, 2009, 37(12) |
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| 作者姓名： | 王磊 樊军锋 刘永红 杨培华 王孟昌 |
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| 作者单位： | 1. 西北农林科技大学,杨陵,712100
2. 西北农林科技大学林学院,杨陵,712100 |
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| 基金项目： | 国家林业公益性科研项目 |
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| 摘 要： | 利用随机微卫星扩增多态性DNA(RMAPD)分子标记技术,对全国范围内的12个天然居群245个个体进行遗传多样性分析,用筛选出的18对引物组合共扩增出303个条带,其中多态性条带229个,多态条带百分率为77.58%.用NTSYS-PC软件中的SIMQUAL程序计算Jaccard遗传相似性系数(genetic similarity,S_G)并聚类.结果表明:245份油松材料间的S_G值变化范围为0.5238～0.9682,平均为0.8141.采用UPGMA法在0.68水平上可将供试材料聚为4类.内蒙古宁城油松、山西地区油松各自聚为一类外,甘肃小陇山地区油松分别于陕西的洋县、留坝、周至、洛南、柞水的油松聚为一类,黄龙、黄陵、府谷的油松聚为一类.表明各居群间存在较高遗传多样性.
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| 关 键 词： | 随机微卫星扩增DNA多态性 随机扩增DNA多态性 油松 多态性 聚类分析 |
Genetic Polymorphism of Pinus tabulaeformis from Main Distribution Areas in China |
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| Wang Lei,Fan Junfeng,Liu Yonghong,Yang Peihua,Wang Mengchang. Genetic Polymorphism of Pinus tabulaeformis from Main Distribution Areas in China[J]. Journal of Northeast Forestry University, 2009, 37(12) |
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| Authors: | Wang Lei Fan Junfeng Liu Yonghong Yang Peihua Wang Mengchang |
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| Affiliation: | Wang Lei,Fan Junfeng,Liu Yonghong,Yang Peihua,Wang Mengchang(College of Forestry,Northwest A&F University,Yangling 712100,P.R.China) |
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| Abstract: | Random Microsatellite Amplified Polymorphic DNA (RMAPD) markers were used to measure genetic diversity and genetic structure of 245 individuals from 12 natural populations of Pinus tabulaeformis Carr.A total of 303 loci were identified from 18 RMAPD primer combinations,among which 229 loci were polymorphic and accounted for 77.58 percent.Jaccard's genetic similarity coefficients were calculated and a dendrogram was generated by unweighted pair group method of arithmetic average using NTSYS-PC.Result showed ... |
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| Keywords: | Random microsatellite amplified polymorphic DNA RAPD Pinus tabulaeformis Genetic diversities Cluster analysis |
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