| 快速提取籽用南瓜基因组DNA方法的研究 |
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| 引用本文： | 赵茜,徐丽珍. 快速提取籽用南瓜基因组DNA方法的研究[J]. 黑龙江农业科学, 2011, 0(10): 14-15 |
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| 作者姓名： | 赵茜 徐丽珍 |
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| 作者单位： | 黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨,150086 |
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| 摘 要： | 为快速高效提取作物基因组DNA,以南瓜品种为试材,对籽用南瓜基因组DNA的快速、高效提取方法进行研究,并对产物进行紫外分光光度计和琼脂糖凝胶电泳分析。结果表明：通过对CTAB改良后提取的基因组DNA结构较完整,杂质较少,浓度较高,可经纯化直接用于进一步的PCR等分子生物学研究。
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| 关 键 词： | 籽用南瓜 DNA 提取方法 |
A Rapid Method for Extraction Genomic DNA from Seed Squash |
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| ZHAO Qian,XU Li-zhen. A Rapid Method for Extraction Genomic DNA from Seed Squash[J]. Heilongjiang Agricultural Science, 2011, 0(10): 14-15 |
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| Authors: | ZHAO Qian XU Li-zhen |
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| Affiliation: | ZHAO Qian,XU Li-zhen(Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086) |
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| Abstract: | In order to extract DNA fast and efficient,the seed squash was taken as materials to study the extraction method.It was analyzed by ultraviolet spectrophotometer and agarose gel electrophoresis.The results showed that structure of DNA was complete but had less impurities,its concentration was high,it could be directly used for the further study of molecular biology(such as PCR). |
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| Keywords: | seed squash DNA extraction method |
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