| 重组山羊FSH基因在毕赤酵母中的高效表达 |
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| 引用本文： | 杨欢利,毛英姿,丁家桐.重组山羊FSH基因在毕赤酵母中的高效表达[J].黑龙江动物繁殖,2011(5):12-15. |
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| 作者姓名： | 杨欢利 毛英姿 丁家桐 |
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| 作者单位： | 1. 浙江省台州医院生殖中心,浙江台州,317000
2. 扬州大学动物科学与技术学院,江苏扬州,225009 |
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| 摘 要： | 为探讨快速高效表达山羊FSH（促卵泡素）基因的方法,将获得的FSHα,β亚基基因克隆到毕赤酵母快速表达载体pPICZαA中,然后将构建的载体线性化处理后,电击转化至毕赤酵母GS115中,经Zeocin抗生素筛选后获得阳性菌落,经甲醇诱导表达后进行SDS-PAGE和Western–blot分析。结果表明：pPICZαA载...
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| 关 键 词： | 山羊 FSH基因 毕赤酵母表达载体 表达 |
High-level Expression of Recombinant Follicle-stimulating Hormone Gene in Pichia pastoris |
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| YANG Huan-li,MAO Ying-zi,DING Jia-tong.High-level Expression of Recombinant Follicle-stimulating Hormone Gene in Pichia pastoris[J].Heilongjiang Journal of Animal Reproduction,2011(5):12-15. |
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| Authors: | YANG Huan-li MAO Ying-zi DING Jia-tong |
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| Institution: | 1.Department of Reproductive Medicine,Taizhou Hospital of Zhejang Province,Taizhou,317000,China; 2.College of Animal Science and Technology,Yangzhou University,Yangzhou,225009,China) |
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| Abstract: | In order to discuss., the methods of expression of goat follicle-stimulating hormone fastly and efficiently.The obtained FSH α, β-subunit genes were cloned into Pichia pastoris pPICZ α A vector,then the recombined vector pPICZ α A FSH or, β were lined and transformed into Pichia pastoris GSl15 by electroporation.The positive Yeast transformants were screened by the medium containedZeocin.After methanol induction,the supernatant was analysised by SDS-Polyacrylamide Gen Electrophoresis and Western-blot.The results show that FSH could be expressed fastly and precisely and the molecular weight is about 27KD.The concentration of supernatant was detected by Radio-immunoassay and the average expression is 20.155mlU/mL ,which is higher than expressed in cells significantly. |
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| Keywords: | goat follicular-stimulating hormone gene Pichia pastoris vector expression |
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