| 利用梯度PCR和降落PCR扩增CIRP基因的比较 |
| |
| 引用本文： | 李玉恒,赵明慧,赵紫阳,李峥,袁雪,李昌盛,林增,胡婧,李俭,李士泽. 利用梯度PCR和降落PCR扩增CIRP基因的比较[J]. 黑龙江八一农垦大学学报, 2011, 23(5): 46-49,75 |
| |
| 作者姓名： | 李玉恒 赵明慧 赵紫阳 李峥 袁雪 李昌盛 林增 胡婧 李俭 李士泽 |
| |
| 作者单位： | 黑龙江八一农垦大学动物科技学院,大庆,163319 |
| |
| 基金项目： | 农业部948重点项目(2011-G35); 2011年黑龙江省研究生创新科研项目(YJSCX2011-267HLJ) |
| |
| 摘 要： | 提取SD大鼠总RNA,同时应用降落PCR和梯度PCR在常规PCR反应体系和低模板浓度PCR反应体系中扩增CIRP基因片段,对两种方法的扩增效果进行比较。结果表明：降落PCR法可以通过一次反应即顺利扩增出CIRP基因特异性条带,并且在模板浓度降低后也能扩增出产物;梯度PCR法扩增结果不稳定,不但有非特异性条带出现而且不能...
|
| 关 键 词： | 降落PCR 梯度PCR 退火温度 CIRP基因 |
Comparison of the Amplification of CIRP Genes with Touch Down PCR and Gradient PCR |
| |
| Li Yuheng,Zhao Minghui,Zhao Ziyang,Li Zheng,Yuan Xue,Li Changsheng,Lin Zeng,Hu Jing,Li Jian,Li Shize. Comparison of the Amplification of CIRP Genes with Touch Down PCR and Gradient PCR[J]. journal of heilongjiang bayi agricultural university, 2011, 23(5): 46-49,75 |
| |
| Authors: | Li Yuheng Zhao Minghui Zhao Ziyang Li Zheng Yuan Xue Li Changsheng Lin Zeng Hu Jing Li Jian Li Shize |
| |
| Affiliation: | Li Yuheng,Zhao Minghui,Zhao Ziyang,Li Zheng,Yuan Xue,Li Changsheng,Lin Zeng,Hu Jing,Li Jian,Li Shize(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319) |
| |
| Abstract: | To extract the total RNA from rats,then to target CIRP genes by touch down PCR(TD-PCR) and gradient PCR(G-PCR) with the popular reaction system and the reaction system content low primer.The results indicated that TD-PCR was efficient in amplifying the CIRP gene from rats and it was more specific than G-PCR.TD-PCR with the same reaction system has higher specificity than G-PCR.It can efficiently improve specificity of PCR and be used to clone gene fragments which are difficult to clone by the G-PCR method. |
| |
| Keywords: | touch down PCR gradient PCR annealing temperature CIRP gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
