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| GA_3诱导时间及诱导后不同培养条件对匍匐剪股颖愈伤组织形成的影响 | |
| 引用本文： | 黄连成,胡鸢雷,邓馨,孙冬梅.GA_3诱导时间及诱导后不同培养条件对匍匐剪股颖愈伤组织形成的影响[J].黑龙江八一农垦大学学报,2011,23(5):9-12. |
| 作者姓名： | 黄连成 胡鸢雷 邓馨 孙冬梅 |
| 作者单位： | 1. 黑龙江八一农垦大学生命科学技术学院,大庆,163319 2. 北京大学生命科学学院 3. 中国科学院植物研究所 |
| 基金项目： | 国家高技术研究发展计划(863计划)重点项目子课题(2007AA021403) |
| 摘 要： | 为了使基因工程中剪股颖愈伤组织发挥更好的效果,以冷季型草坪草剪股颖的成熟种子为研究材料,通过改变不同培养基添加物、添加不同质量浓度的2,4-D（2,4-二氯苯氧乙酸）等,研究了在不同培养条件下GA3诱导对剪股颖愈伤组织形成的影响。结果表明：不论在何种培养条件下,均以经50 mg.L-1 GA3溶液中浸泡4 d的愈伤组织... |
| 关 键 词： | 剪股颖 GA处理 愈伤组织 培养条件 |
Different Induction Time and Culture Condition Effect on Cold-Season Creeping Bentgrass Callus Formation |
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| Huang Liancheng,Hu Yuanlei,Deng Xin,Sun Dongmei.Different Induction Time and Culture Condition Effect on Cold-Season Creeping Bentgrass Callus Formation[J].Journal of Heilongjiang August First Land Reclamation University,2011,23(5):9-12. | |
| Authors: | Huang Liancheng Hu Yuanlei Deng Xin Sun Dongmei |
| Institution: | Huang Liancheng1,Hu Yuanlei2,Deng Xin3,Sun Dongmei1(1.College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,2.College of Life Science,Peking University,3.Institute of Botany,Chinese Academy of Science) |
| Abstract: | To improve the efficiency of callus formation of the cold-season creeping bentgrass,induction experiments were performed under the condition of changing 2,4-D concentration and adding different additives in the media.The results showed that the optimal callus formation condition was soaking the seeds in 50 mg·L-1 GA3 solution for 4 days under the different culture conditions.The optimal 2,4-D concentration for callus induction was 5.0 mg·L-1,adding proline or /and casein hydrolysate in the culture medium can considerably promote the callus formation ratio of creeping bentgrass. |
| Keywords: | creeping bentgrass GA3 treatment callus culture condition |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |