日粮蛋白质水平对湖羊胃肠道pH、氨态氮及尿素氮的影响

本试验用装有永久性瘤胃瘘管、十二指肠近端T型瘘管的3只湖羊(35±5)kg作为试验动物,按3×3拉丁方试验设计方案,研究了日粮蛋白质水平分别为8.4%(A)、11.2%(B)、14.0%(C)时对湖羊瘤胃和十二指肠pH值、氨态氮、尿素氮浓度的影响。结果表明:①瘤胃pH为6.31~6.43,各组间差异不显著(P>0.05);十二指肠pH为2.64~2.70,各组间差异不显著(P>0.05)。②瘤胃氨态氮浓度(3.24~11.99)mg/100mL,C组极显著高于A、B组,B组极显著高于A组(P<0.01);十二指肠氨态氮浓度(3.01~7.88)mg/100mL,C组极显著高于A、B组,B组极显著高于A组(P<0.01)。③瘤胃尿素氮浓度(13.81~17.51)mg/100mL,C组极显著高于A、B组(P<0.01),B组显著高于A组(P<0.05);十二指肠尿素氮浓度(4.1~5.91)mg/100mL,B、C组极显著高于A组(P<0.01),B组与C组差异不显著(P>0.05)。

Effects of different dietary protein level on pH,NH3-N and urea-N in gastrointestinal tract of hu sheep

Three Hu sheep(35±5)kg fitted with permanent ruminal cannulas,proximal duodenal T-shaped cannulas were used to investigate the effects of three different dietary protein levels on pH,NH_3-N and Urea-N in gastrointestinal tract.The method of 3×3 Latin square design was applied with three treatments for CP concentration in diets as 8.4%(A),11.2%(B) and 14.0%(C),respectively.The result showed: ①Average pH values in rumen and duodenum varied between 6.31～6.43 and 2.64～2.70,respectively,and the different level of CP in diet had no significant effects on the gastrointestinal pH.②The content of NH_3-N both in Rumen and duodenum changed between(3.24～11.99)mg/100 mL and(3.01～7.88) mg/100 mL respectively,and the concentration of NH_3-N in gastrointestinal tract in C group was higher than A and B groups,while A group was lowest(P<0.01).③Ruminal Urea-N ranged between(13.81～17.51) mg/100 mL,the concentration of Urea-N in C group was highest,lowest in A and middle in B.Duodenal Urea-N was(4.1～5.91) mg/100 mL,the concentration of Urea-N in B and C groups were extremely higher than that in A(P<0.01),but no significant effect occurred between B and C groups(P>0.05).